The Duolink PLA red mouse/rabbit kit was used to perform proximity ligation assay per manufacturer’s protocols. Briefly, cells were plated in 8-well chamber slides overnight. The next day, media was aspirated, cells were fixed with 4% formaldehyde with methanol permeabilization. Cells were then blocked with Duolink blocking solution and incubated with primary antibody overnight. Next, Duolink PLA probes were incubated with the samples followed by ligation and amplification. Finally, the slides were mounted with a coverslip using Duolink in situ mounting medium with DAPI, and the slides were either imaged 15 minutes later or kept at −20°C until imaging using a Leica SP5 Inverted Confocal Microscope (DMI6000CS) with the 405 nm and 647 nm lasers for excitation.
Sp5 inverted confocal microscope dmi6000cs
Manufactured by Leica
The SP5 Inverted Confocal Microscope (DMI6000CS) is a high-performance, inverted microscope system designed for advanced imaging and analysis. It features a fully integrated confocal scanning system, enabling high-resolution, optical sectioning of samples. The SP5 is equipped with a flexible, modular design to accommodate a wide range of microscopy techniques and applications.
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Lab products found in correlation
2 protocols using sp5 inverted confocal microscope dmi6000cs
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Proximity Ligation Assay for Protein Interactions
2
Proximity Ligation Assay for Protein Interactions
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The Duolink PLA red mouse/rabbit kit was used to perform proximity ligation assay per manufacturer's protocols. Briefly, cells were plated in 8-well chamber slides overnight. The next day, media was aspirated, cells were fixed with 4% formaldehyde with methanol permeabilization. Cells were then blocked with Duolink blocking solution and incubated with primary antibody overnight. Next, Duolink PLA probes were incubated with the samples followed by ligation and amplification. Finally, the slides were mounted with a coverslip using Duolink in situ mounting medium with DAPI, and the slides were either imaged 15 minutes later or kept at -20°C until imaging using a Leica SP5 Inverted Confocal Microscope (DMI6000CS) with the 405 nm and 647 nm lasers for excitation.
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