Modular dpp

Manufactured by Roche

Sourced in Germany

The Modular DPP is a versatile laboratory equipment designed for performing a variety of diagnostic and analytical tasks. It offers a modular and automated platform for efficient sample processing and analysis. The core function of the Modular DPP is to provide a reliable and streamlined solution for various laboratory applications.

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Lab products found in correlation

Dia mat glycosylated hemoglobin analyzer system, by Bio-Rad (1 mentions) Colorimetric diagnostic kit, by Applygen (1 mentions) Immulite 2000 double system platform, by Siemens (1 mentions) Cobas 6000, by Roche (1 mentions) Symbia t2, by Siemens (1 mentions)

Spelling variants of this product

Modular DPP system (12 citations) Modular DPP H7600 (5 citations) Modular DPP Automatic Biochemical Analyzer (2 citations) DPP Modular analyzer (2 citations)

12 protocols using modular dpp

Anthropometric Measurements and Metabolic Markers

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Anthropometric measurements including height, weight, waist and hip circumferences, and systolic and diastolic blood pressures were measured using standardized procedures. Waist-hip ratio was calculated as waist circumference (cm) divided by hip circumference (cm). Body mass index (BMI) was calculated as body weight in kilograms divided by the square of height in meters. The homeostasis model assessment (HOMA) was used to assess individual insulin resistance (HOMA-IR). HOMA-IR = (FPG [mmol/L] × FIN [mU/L])/22.5, FIN represents fasting insulin. HOMA-β was used to assess islet beta-cell secretion function. HOMA-β = FIN × 20/(FPG − 3.5).[14 (link)15 (link)]
Peripheral venous blood samples were collected in tubes from all participants during the fasting state. Plasma insulin levels were measured by double-antibody radioimmunoassay. FPG was quantified by the glucose oxidase-peroxidase procedure (Modular DPP, Roche Diagnostics GmbH, Mannheim, Germany). Serum total cholesterol (TC), triglycerides (TGs), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein (LDL) levels were measured using an automatic biochemical analyzer (Modular DPP, Roche Diagnostics GmbH). HbA1c levels were measured using a high-performance liquid chromatography system (Bio-Rad DIA-MAT glycosylated hemoglobin analyzer system, Bio-Rad, Hercules, CA, USA).

Sun L.L., Zhang S.J., Chen M.J., Elena K, & Qiao H. (2017). Relationship between Modulator Recognition Factor 2/AT-rich Interaction Domain 5B Gene Variations and Type 2 Diabetes Mellitus or Lipid Metabolism in a Northern Chinese Population. Chinese Medical Journal, 130(9), 1055-1061.

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Serum Biomarker Analysis in Burn Patients

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The activity levels of aspartate aminotransferase (AST), alanine transaminase (ALT), lactate dehydrogenase (LDH), and TG levels in the serum and cell culture supernatants were measured using an auto-analyzer (Modular DPP; Roche, Switzerland).
Serum concentrations of carnitine, β-HB, and ornithine carbamoyltransferase (OCT) in the model rats and human burn patients were determined using Immunoassay Kits (Jiancheng Bioengineering Institute of Nanjing, Nanjing, China) according to the manufacturer’s instructions.

Li P., Xia Z., Kong W., Wang Q., Zhao Z., Arnold A., Xu Q, & Xu J. (2021). Exogenous L-carnitine ameliorates burn-induced cellular and mitochondrial injury of hepatocytes by restoring CPT1 activity. Nutrition & Metabolism, 18, 65.

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Serum Biomarker Profiling in Liver

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Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), glucose, TG and total cholesterol (TC) levels were assessed with an automated analyzer (Roche Modular DPP). TG and TC levels in liver samples were assessed with a colorimetric diagnostic kit (Applygen Technologies, Inc, Beijing, China) based on provided directions. Final TG and TC concentrations were adjusted based on protein content levels.

Wang D., Si D., Li G., Ding Z., Yang X, & Gao C. (2022). Dysregulated autophagic activity induced in response to chronic intermittent hypoxia contributes to the pathogenesis of NAFLD. Frontiers in Physiology, 13, 941706.

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Serum Biomarker Analysis Protocol

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Blood samples collected at selected time points were divided into 2 groups. Samples from one group were kept at room temperature for 2 h and then at 4 °C overnight, with the sera collected and frozen. Sera from the groups were thawed and centrifuged at 3000 rpm for 10 min prior to testing. Serum levels of AST, ALT and TBIL were then tested with a Roche Modular DPP automatic biochemical analyser (facilitated in the 2nd Affiliated Hospital of Harbin Medical University). Samples in the other group were stored at 4 °C in a refrigerator overnight and then centrifuged at 1000 × g for 20 min. Serum showing lysis of the red blood cells was excluded from the study. Total collagen content was detected by measuring the hydroxyproline level in serum samples using a Hydroxyproline Assay Kit (CEA621Ge 96T, Cloud-Clone Corp) following the manufacturer’s instructions.

Zhang Y., Li R., Rong W., Han M., Cui C., Feng Z., Sun X, & Jin S. (2018). Therapeutic effect of hepatocyte growth factor-overexpressing bone marrow-derived mesenchymal stem cells on CCl4-induced hepatocirrhosis.. Cell Death & Disease, 9(12), 1186.

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Fasting Blood Lipid Profiling

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Blood samples were taken by puncturing the cubital vein, under standardized conditions, between 8:15 and 9:00 A.M., with the participant having fasted at least 12 hours beforehand. When the transfer of the samples to the laboratory took longer than 75 min, they were centrifuged in situ and transferred refrigerated.
The following variables were determined according to biochemical parameters: triglycerides (TGs) (glycerol phosphate oxidase-peroxidase [GPO-PAP] enzymatic method) and c-direct plus high-density lipoprotein (HDL). Lipid profile determinations were made on a weekend, in a MODULAR DPP from Roche Diagnostics, and insulin levels were assessed using an Immulite 2000 double system platform of Siemens.

Lucas-de la Cruz L., Martín-Espinosa N., Cavero-Redondo I., González-García A., Díez-Fernández A., Martínez-Vizcaíno V, & Notario-Pacheco B. (2018). Sleep patterns and cardiometabolic risk in schoolchildren from Cuenca, Spain. PLoS ONE, 13(1), e0191637.

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Bovine Serum Calcium Profiling

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Blood samples (10 mL) from all tested cows were collected from the caudal vein at three time points in accordance with the International Guiding Principles for Biomedical Research Involving Animals. The samples were centrifuged immediately at 4,000 g for 10 min. Then, the serum fraction was frozen in liquid nitrogen and stored at −80°C until subsequent analyses. The serum Ca concentration was detected using a commercial kit (651564-01; Roche Diagnostics, Mannheim, Germany) with an automatic biochemical analyzer (Modular DPP; Roche Diagnostics, Germany). The serum Ca concentrations at the three time points are shown Figure 1 and group values are shown in Supplementary Table S1.
To reduce individual differences, every sample for 2D-DIGE was obtained from serum samples of three animals. The test design is depicted in Table 1 and the study strategy is shown in Supplementary Table S2.

Shu S., Bai Y., Wang G., Xiao X., Fan Z., Zhang J., Zhao C., Zhao Y., Xia C, & Zhang H. (2016). Differentially expressed serum proteins associated with calcium regulation and hypocalcemia in dairy cows. Asian-Australasian Journal of Animal Sciences, 30(6), 893-901.

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Comprehensive Lipid and Liver Panel

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Serum triglyceride (TG), total cholesterol (CHOL), high-density lipoprotein cholesterol (Chol-HDL), low-density lipoprotein cholesterol (Chol-LDL), very low-density lipoprotein cholesterol (Chol-VLDL), alanine transaminase (ALT), and aspartate aminotransferase (AST) were determined by routine laboratory methods using an autoanalyzer (Roche, Modular DPP, NO.1549-06).

Hu C.Q., Bo Q.L., Chu L.L., Hu Y.D., Fu L., Wang G.X., Lu Y., Liu X.J., Wang H, & Xu D.X. (2020). Vitamin D Deficiency Aggravates Hepatic Oxidative Stress and Inflammation during Chronic Alcohol-Induced Liver Injury in Mice. Oxidative Medicine and Cellular Longevity, 2020, 5715893.

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Automated Plasma Calcium Measurement

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The plasma Ca concentration was measured using an automatic biochemical analyser (modular DPP, Roche Diagnostics GmbH) and a Ca plasma kit (651564-01, Roche Diagnostics GmbH).

Fan Z., Shu S., Xu C., Xiao X., Wang G., Bai Y., Xia C., Wu L., Zhang H., Xu C, & Yang W. (2017). Protein profiling of plasma proteins in dairy cows with subclinical hypocalcaemia. Irish Veterinary Journal, 70, 3.

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Demographic and Metabolic Biomarker Profiling

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Information on demographic characteristics and health-related history was obtained using self-reported questionnaires. The BMI was calculated as weight in kilogram divided by height in meters squared and then rounded to one decimal place. Blood samples were collected after an overnight fast. FPG concentration was determined on an automated chemistry analyzer (Hitachi Modular DPP, Roche Diagnostics, Tokyo, Japan) using a hexokinase method. HbA1c was measured using a non-porous ion-exchange liquid chromatography with a HLC-723 Tosoh G8 automatic analyzer (Tosoh Corporation, Tokyo, Japan).

Han J.M., Cho J.H., Kim H.I., Suh S., Lee Y.J., Lee J.W., Kim K.M, & Bae J.C. (2023). Greater Severity of Steatosis Is Associated with a Higher Risk of Incident Diabetes: A Retrospective Longitudinal Study. Endocrinology and Metabolism, 38(4), 418-425.

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Comprehensive Thyroid Disorder Evaluation

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Serum levels of ALT (normal range: 5–35 IU/L), AST (normal range: 5–32 IU/L), ALP (normal range: 40–150 IU/L), γ-GTP (normal range: 5–36 IU/L), TBIL (normal range: 0–17.1 μmol/L), DBIL (normal range: 0–6.8 μmol/L) were measured using a routine automated analyzer (Modular DPP, Roche Diagnostics GmbH, Mannheim, Germany). Serum FT3 (normal range: 3.1–6.8 pmol/L) and FT4 (normal range: 12–22 pmol/L) concentrations, TRAb levels (normal range: 0–1.75 IU/L), anti-TPO (normal range: 0–34 IU/L) and anti-Tg (normal range: 0–115 IU/L) values were measured by chemiluminescent immunoassays (Cobas 6000, Roche Diagnostics GmbH, Mannheim, Germany).
The BMI was calculated as [mass (kg)]/[height (m)]^{2 (link)}. The 24 h-RAIU value was obtained 24 h after an oral tracer dose (approximately 74 kBq) of 131-radioiodine was administered using a thyroid function instrument (HH-6008, Beijing Hehai Advanced Technology, China). A thyroid scan was performed using 99mTc-pertechnetate via a dual-detector variable-angle gamma camera coupled with a 2-slice CT scanner (Symbia T2, Siemens, Munich, Germany), and thyroid weight was obtained automatically when the region of interest, long and short axis of the thyroid gland were outlined.

Zhang R., Tian X., Qin L., Wei X., Wang J, & Shen J. (2015). Factors Predicting Abnormal Liver Function Tests Induced by Graves’ Disease Alone: A Retrospective Cohort Study. Medicine, 94(19), e839.

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