Rabbit anti il 1β sc 7884

Immunohistochemistry was performed as previously described [6 (link)]. In brief, 5-μm-thick sections (3 sections per specimen) were prepared according to standard protocols for HE staining. Rabbit anti-IL-1β (sc-7884, Santa Cruz, USA), Rabbit anti-TNF-α (ab6671, Abcam, Hong Kong), and Rabbit anti-TREM1 (AF8226, Beyotime, Shanghai) served as the primary antibodies, and goat anti-rabbit horseradish peroxidase–conjugated IgG served as the secondary antibody. The slides were incubated with primary antibodies overnight at 4 °C and with secondary antibodies for 30 min at 37 °C. Diaminobenzidine (SP immunochemistry kit; Boster Biological Technology, Ltd.) was then applied, followed by counterstaining with hematoxylin. Image acquisition was performed using the same method used for the HE-stained sections. The immunohistochemical measurements were taken by 2 observers. The sections were scored according to the intensity of the stain in the condyle (0, negative, no staining; 1, faint yellow, mild staining; 2, clay bank, moderate staining; and 3, brown, intense staining). Interobserver differences were reconciled through microscope conferencing.