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Sorafenib s7397

Manufactured by Selleck Chemicals
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Sorafenib (S7397) is a lab reagent manufactured by Selleck Chemicals. It is a small molecule that functions as a multi-kinase inhibitor.

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Lab products found in correlation

5 fu s1209, by Selleck Chemicals (1 mentions) Dox s1208, by Selleck Chemicals (1 mentions) Anti cleaved caspase 3 9661, by Cell Signaling Technology (1 mentions) Anti β actin a1978, by Merck Group (1 mentions) Cisplatin s1166, by Selleck Chemicals (1 mentions) Cck 8 assay kit, by Enzo Life Sciences (1 mentions) Oligonucleotide primers, by Integrated DNA Technologies (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Streptomycin sulfate, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Sorafenib (230 citations) Sorafenib (No. S7397), (2 citations)

4 protocols using sorafenib s7397

1

Evaluating HCC Drug Sensitivity

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Two 3D-HCC cancer in vitro models (CSM and ASM) were used in drug sensitivity assays to identify changes in drug sensitivity for each model. The drugs most commonly used for HCC were selected. Sorafenib (S7397), Cabozantinib (S1119), Lenvatinib (S1164), Regorafenib (S1178), 5-FU (S1209), and DOX (S1208) were purchased from Selleckchem (Houston, TX, USA). Drugs were dissolved in a stock solution of dimethyl sulfoxide (DMSO, 100 mM).
Lee S.Y., Teng Y., Son M., Ku B., Hwang H.J., Tergaonkar V., Chow P.K., Lee D.W, & Nam D.H. (2021). Three-Dimensional Aggregated Spheroid Model of Hepatocellular Carcinoma Using a 96-Pillar/Well Plate. Molecules, 26(16), 4949.
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2

Cytotoxicity Assays and Reagents

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CCK-8 assay kit (ALX-850–039) was purchased from Enzo Life Sciences (Farmingdale, NY). 5-fluorouracil (5-FU, F6627), doxorubicin (D1515) and ketone body assay kit (MAK134) were obtained from Sigma-Aldrich (St. Louis, MO). Cisplatin (S1166) and sorafenib (S7397) were from Selleck Chemicals (Houston, TX). The antibodies used in this study included anti-SLC13A5 (ab89181) from Abcam, anti-cleaved Caspase-3 (9661S) from Cell Signaling Technology and anti-β-actin (A1978) from Sigma-Aldrich. Oligonucleotide primers were synthesized by Integrated DNA Technologies (Coralville, IA). Unless otherwise specified, all cell culture reagents were purchased from Sigma-Aldrich or Life Technologies.
Hu T., Huang W., Li Z., Kane M.A., Zhang L., Huang S.M, & Wang H. (2020). Comparative proteomic analysis of SLC13A5 knockdown reveals elevated ketogenesis and enhanced cellular toxic response to chemotherapeutic agents in HepG2 cells. Toxicology and applied pharmacology, 402, 115117.
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3

Huh7 and Hep3B Cell Line Culture

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Huh7 and Hep3B cell lines were purchased from the National Collection of Authenticated Cell Cultures (Shanghai, China) and were cultured in complete medium containing Dulbecco’s modified Eagle’s medium (HyClone, Logan, UT, USA) supplemented with 10% fetal bovine serum (Gibco, Grand Island, NY, USA), 1000 U/mL penicillin, and 100 μg/mL streptomycin (HyClone, Logan, UT, USA), and were grown in a humidified air atmosphere containing 5% CO2 at 37 °C. All cell lines were analyzed by STR profiling for cell line authentication and routine mycoplasma detection. Sorafenib (S7397) was purchased from Selleckchem (Houston, TX, USA).
Jiang C., Liu Z., Yuan J., Wu Z., Kong L., Yang J, & Lv T. (2023). Construction of Two Independent RAB Family-Based Scoring Systems Based on Machine Learning Algorithms and Definition of RAB13 as a Novel Therapeutic Target for Hepatocellular Carcinoma. International Journal of Molecular Sciences, 24(5), 4335.
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4

Antibody-based Signaling Pathway Analysis

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Antibodies were from Abcam PLC (UK) and included rabbit anti-CBL (ab32027), anti-CBL (phosphoY731) (ab52855), anti-c-Kit (ab32363), anti-P-glycoprotein (ab170904), anti-PDGFR-beta (ab32570), anti-ERK1/2 (ab223500) and anti-beta-actin (ab8227); rabbit anti-Akt (9272), anti-p308-Akt (13038) and anti-p-ERK1/2 (4370s) were from CST, Inc. (USA). The horseradish peroxidase (HRP)-conjugated secondary antibodies (111-001-003) were from Jackson IR (USA); SCF (RP8631) was from Invitrogen, Inc. (USA). Sorafenib (S7397) was from Selleck Chemicals (USA), and cytarabine (cytosar) was from Pfizer, Inc. (USA). DMEM, RPMI-1640 medium, fetal bovine serum (FBS), penicillin, and streptomycin sulfate were obtained from Invitrogen, Inc. (USA).
Sun Q., Wu B., Meng F., Yao Q., Huang Z., Xu J, & Zhu Z. (2023). Sorafenib regulates c-CBL gene-mediated chemoresistance in acute myeloid leukemia cells. Hematology (Amsterdam, Netherlands), 28(1).
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