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Anti cd19 pe

Manufactured by Beckman Coulter
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Anti-CD19-PE is a fluorochrome-conjugated antibody that targets the CD19 antigen, which is expressed on the surface of B lymphocytes. It is commonly used in flow cytometry applications to identify and quantify B cells within a sample.

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Lab products found in correlation

Anti cd14 pe texas red, by Beckman Coulter (2 mentions) Anti cd38 pe cy7, by Beckman Coulter (2 mentions) Anti cd45 apc, by Beckman Coulter (2 mentions) Anti cd33 pe cy5, by Beckman Coulter (2 mentions) Anti cd15 pacific blue, by Beckman Coulter (2 mentions) Anti cd3 fitc, by Beckman Coulter (2 mentions) Anti calnexin, by Cell Signaling Technology (1 mentions) Anti calreticulin, by Cell Signaling Technology (1 mentions) Anti pdi, by Cell Signaling Technology (1 mentions) Anti cd19, by Cell Signaling Technology (1 mentions) Alexa fluor 594, by Thermo Fisher Scientific (1 mentions) Anti vsv g, by Abcam (1 mentions) Anti cd3 apc, by Beckman Coulter (1 mentions) Anti cd20 fitc, by Beckman Coulter (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Anti cd4 fluorescein isothiocyanate fitc, by Thermo Fisher Scientific (1 mentions) Anti cd38 pe cy5, by BioLegend (1 mentions) Fixation permeabilization buffer solution, by Thermo Fisher Scientific (1 mentions) Anti cd25 pe cy5, by Thermo Fisher Scientific (1 mentions) Accuri c6 flow cytometer, by BD (1 mentions)

5 protocols using anti cd19 pe

1

Engraftment of AML in NSG Mice

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Animal experiments were performed in accordance with institutional guidelines approved by the UHN Animal Care Committee. 8 to 12-week-old female NOD/SCID/IL-2Rgc-null (NSG) mice were sublethally irradiated (225 cGy) 6–24 hours before transplantation. Mononuclear cells from AML patients were depleted of CD3+ cells by EasySep (Stem Cell Technologies) prior to intrafemoral transplantation. Mice were sacrificed 8 or 16 weeks after transplantation and human engraftment in the injected femur and non-injected bone marrow was evaluated by flow cytometry using the following human-specific antibodies (all used at 1:200, all from BD unless stated otherwise, catalogue number in parentheses): anti-CD45-APC (340943), anti-CD19-PE, anti-CD33-PE-Cy5, anti-CD3-FITC, anti-CD14-PE Texas Red (Beckman Coulter PNIM2707U), anti-CD15-Pacific Blue (642917), anti-CD38-PE-Cy7, and anti-CD34-APC-Cy7. The threshold for detection of human engraftment was 0.1% CD45+ cells. All flow cytometric analysis was performed on the LSRII (BD Biosciences). For limiting dilution assays, the frequency of repopulating cells was calculated using ELDA software49 (link).
Shlush L.I., Zandi S., Mitchell A., Chen W.C., Brandwein J.M., Gupta V., Kennedy J.A., Schimmer A.D., Schuh A.C., Yee K.W., McLeod J.L., Doedens M., Medeiros J.J., Marke R., Kim H.J., Lee K., McPherson J.D., Hudson T.J., Brown A.M., Trinh Q.M., Stein L.D., Minden M.D., Wang J.C, & Dick J.E. (2014). Identification of pre-leukemic hematopoietic stem cells in acute leukemia. Nature, 506(7488), 328-333.
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2

Engraftment of AML in NSG Mice

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Animal experiments were performed in accordance with institutional guidelines approved by the UHN Animal Care Committee. 8 to 12-week-old female NOD/SCID/IL-2Rgc-null (NSG) mice were sublethally irradiated (225 cGy) 6–24 hours before transplantation. Mononuclear cells from AML patients were depleted of CD3+ cells by EasySep (Stem Cell Technologies) prior to intrafemoral transplantation. Mice were sacrificed 8 or 16 weeks after transplantation and human engraftment in the injected femur and non-injected bone marrow was evaluated by flow cytometry using the following human-specific antibodies (all used at 1:200, all from BD unless stated otherwise, catalogue number in parentheses): anti-CD45-APC (340943), anti-CD19-PE, anti-CD33-PE-Cy5, anti-CD3-FITC, anti-CD14-PE Texas Red (Beckman Coulter PNIM2707U), anti-CD15-Pacific Blue (642917), anti-CD38-PE-Cy7, and anti-CD34-APC-Cy7. The threshold for detection of human engraftment was 0.1% CD45+ cells. All flow cytometric analysis was performed on the LSRII (BD Biosciences). For limiting dilution assays, the frequency of repopulating cells was calculated using ELDA software49 (link).
Shlush L.I., Zandi S., Mitchell A., Chen W.C., Brandwein J.M., Gupta V., Kennedy J.A., Schimmer A.D., Schuh A.C., Yee K.W., McLeod J.L., Doedens M., Medeiros J.J., Marke R., Kim H.J., Lee K., McPherson J.D., Hudson T.J., Brown A.M., Trinh Q.M., Stein L.D., Minden M.D., Wang J.C, & Dick J.E. (2014). Identification of pre-leukemic hematopoietic stem cells in acute leukemia. Nature, 506(7488), 328-333.
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3

Comprehensive Antibody Detection Protocol

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The following antibodies were used for protein detection: anti-CD19–PE (Beckman Coulter; IM1285U), anti-CD19 (Cell Signaling; 3574), anti-CD19 3G7 (Origen; TA506234), anti-CD19 HD37 (Thermo Fisher; MA1-34005), anti-VSVg–fluorescein isothiocyanate (FITC) (Abcam; ab3863), anti-VSVg (Abcam; ab50549), anti-CD81 (LifeSpan; LS-C359239), anti-CD81–FITC (Molecular Probes; A15753), anticalnexin (Cell Signaling; 2679), anticalreticulin (Cell Signaling; 12238), anti-UGCGL1 (NovoPro Laboratories; 116554), anti-PDI (Cell Signaling; 3501), anti-mouse antibody–Alexa Fluor 488 (Thermo Fisher; R37114), and anti-rabbit antibody–Alexa Fluor 594 (Thermo Fisher; A-21207).
Bagashev A., Sotillo E., Tang C.H., Black K.L., Perazzelli J., Seeholzer S.H., Argon Y., Barrett D.M., Grupp S.A., Hu C.C, & Thomas-Tikhonenko A. (2018). CD19 Alterations Emerging after CD19-Directed Immunotherapy Cause Retention of the Misfolded Protein in the Endoplasmic Reticulum. Molecular and Cellular Biology, 38(21), e00383-18.
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4

Multicolor Flow Cytometry for B and T cells

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PBMC were stained with anti-CD20 FITC (Beckman), anti-CD19 PE (Beckman), and anti-CD3 APC (Beckman); dead cells were labeled with DAPI (Invitrogen). Cytometry gating was performed first on lymphocytes, then live cells (DAPI-negative), then on either CD19+ or CD3+, and lastly on CD20+ among CD19+ or CD3+ cells.
Palanichamy A., Jahn S., Nickles D., Derstine M., Abounasr A., Hauser S.L., Baranzini S.E., Leppert D, & von Büdingen H.C. (2014). Rituximab efficiently depletes increased CD20 expressing T cells in multiple sclerosis patients. Journal of immunology (Baltimore, Md. : 1950), 193(2), 580-586.
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5

Phenotyping of Regulatory T and B Cells

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Approximately 1x10 6 PBMC were deposited into flow cytometry tubes (Falcon, Becton Dickinson, Oxnard, California). To identify Treg cells, surface labeling was performed with anti-CD4-fluorescein isothiocyanate (FITC) (clone: OKT4, eBioscience, San Diego, CA) and anti-CD25-PECy5 (clone: BC96 eBioscience) antibodies. The cells were fixed with 2% paraformaldehyde, permeabilized with the fixation/permeabilization buffer solution (eBioscience, San Diego, CA) according to the manufacturer's recommendations, and subsequently incubated with anti-FOXP3-PE antibody (clone: 259D Biolegend). Finally, the cells were washed and resuspended in phosphate-buffered saline (PBS) and read on an Accuri C6 flow cytometer (BD Biosciences). For the phenotyping of the Breg cells, anti-CD19-PE (Beckman Coulter, Brea, CA, USA), anti-CD24-Alexa-fluor-647 (clone: ML5 BioLegend) and anti-CD38-PECy5 (clone: HIT2 BioLegend) antibodies were added for 30 minutes at room temperature.
Subsequently, the cells were washed with PBS and fixed with 2% paraformaldehyde. The samples were read in a flow cytometer.
Burbano Y.C.B., Paz A.V.C., Caldon C.C.R., Constain J.S.R., Gonzáles G.I.Á., Hernández J.C.K., Marin-Agudelo N., Dueñas-Cuellar R.A, & Castaño V.E.N. (2019). Low Frequency Of Regulatory B-Cells And Increased CD4+ and CD8+ Interferon-γ-producing cells in patients with tropical spastic paraparesis associated with human T-cell lymphotropic virus type. Revista da Sociedade Brasileira de Medicina Tropical, 52.
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