Anti ve cadherin f 8

Anti-VE-cadherin (F-8, 1 µg/ml) and DAPI were from Santa Cruz Biotechnology. Anti-E-cadherin (ab1416, 1 µg/ml), anti-Ki67 (ab15580, 0.25 µg/ml), and anti-β tubulin (ab6046, 0.25 µg/ml) were from Abcam. Anti-GM130 (clone 35, 2 µg/ml) was from BD Biosciences. Anti-VEGFA (VG-1, 2 µg/ml), anti-ZO-1 (40-2200, 1 µg/ml), rhodamine phalloidin (1 µg/ml), 70 kDa FITC-dextran and AlexaFluor 647 conjugated goat secondary antibodies were from Life Technologies. Anti-α6 integrin (MA6, 1 µg/ml) was from Millipore. Anti-HA (6E2, 0.5 µg/ml), anti-GFP (D5.1, 0.5 µg/ml), and anti-GAPDH (D16H11, 0.25 µg/ml) were from Cell Signaling Technologies. HRP-conjugated donkey anti-mouse and rabbit IgG secondary antibodies (1: 0.25 µg/ml) were purchased from Fitzgerald. Recombinant human IL-6 protein (7270-IL, 200 ng/ml), recombinant human TGFβ1 protein (240-B, 5 ng/ml), recombinant human FGF2 protein 2 (33-FB, 3 nM), anti-IL-6 (6708, 1 µg/ml), anti-IL-6Rα (MAB227, 0.5 µg/ml), and Proteome Profiler Human Cytokine Array Kit were purchased from R&D Systems. Semaxanib was purchased from Selleckchem.

Purified anti-mouse CD16/32 antibody (Cat. no: 101312, Lot B298973), Alexa Fluor 488 conjugated anti-mouse Ly6G Ab (Cat. no: 127626, Lot B287100), PE conjugated anti-mouse Ly6G Ab (Cat. no: 127608, Lot B290416), Alexa Fluor 488 conjugated Rat IgG2a, κ Isotype control antibody (Cat. no: 400525, Lot B228070), PE conjugated Rat IgG2b, κ Isotype control antibody (Cat. no: 400608, Lot B170512), PE conjugated Mouse IgG1, κ Isotype control antibody (Cat. no: 400139, Lot B244868), PerCP/Cyanine5.5 Rat IgG2a, κ Isotype control antibody (Cat. no: 400531, Lot B268003) were purchased from Biolegend (San Diego, CA). PE conjugated anti-mouse ICAM-1(G-5) Ab (Cat. no: sc-8439; Santa Cruz Biotechnology, Santa Cruz, CA). Anti-VE-cadherin (F-8) (Cat. no: sc-9989 Lot J0217) (Santa Cruz Biotechnology, Santa Cruz, CA). Anti-VE-cadherin (D87F2) (Cat. no: 2500) (Cell Signaling Technology, Danvers, MA). PE Rat anti-mouse CD11b (Cat. no: 557397, Lot 7198506, BD Biosciences, San Jose, CA). APC-conjugated anti-mouse JAM-C(CD323) Rat IgG2b (Cat. no: FAB7050A, Lot ACHX0220011, R&D Systems, Minneapolis, MN). Alexa Fluor 568 goat anti-mouse IgG(H + L) (Cat. no: A11031, Lot 2216598, Invitrogen, Carlsbad, CA).