Abi 3130xl analyser

Manufactured by Thermo Fisher Scientific

Sourced in United States

The ABI 3130xl Genetic Analyzer is a capillary electrophoresis instrument designed for DNA sequencing and fragment analysis. It features 16 capillaries and supports a range of applications, including Sanger sequencing, microsatellite analysis, and SNP genotyping.

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Lab products found in correlation

Genemapper v4, by Thermo Fisher Scientific (1 mentions)

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ABI 3130XL (189 citations) ABI 3130xl Genetic Analyser (48 citations) ABI PRISM 3130xl Genetic Analyser (34 citations) ABI 3130xl DNA analyser (5 citations)

2 protocols using abi 3130xl analyser

Genotyping Candida Species by Microsatellites

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Isolates were genotyped by species-specific microsatellite markers. Six markers were used for C. albicans: CDC3, EF3, HIS3 (Botterel et al., 2001 (link); Sabino et al., 2010 (link)), CAI, CAIII, and CAVI (Sampaio et al., 2005 (link)). Markers used for C. tropicalis were Ctrm1, Ctrm10, Ctrm12, Ctrm21, Ctrm24, and Ctrm28 (Wu et al., 2014 (link)), and CP1, CP4a, CP6, and B were used for C. parapsilosis (Sampaio et al., 2003 (link); Vaz et al., 2011 (link)).
Capillary electrophoresis using the ABI 3130xl analyser (Applied Biosystems-Life Technologies Corporation, Carlsbad, California, USA) and the GeneScan ROX 500 bp marker (Applied Biosystems-Life Technologies Corporation, Carlsbad, California, USA) was performed on the PCR products. Electropherograms were analyzed with the GeneMapper® v.4.0 software (Applied Biosystems-Life Technologies Corporation, California). A control strain from each species was used in each run to ensure size accuracy and avoid run-to-run variations. The number of base pairs determined the size of alleles in each locus.

Guinea J., Arendrup M.C., Cantón R., Cantón E., García-Rodríguez J., Gómez A., de la Pedrosa E.G., Hare R.K., Orden B., Sanguinetti M., Pemán J., Posteraro B., Ruiz-Gaitán A., Parisi G., Da Matta D.A., Colombo A.L., Sánchez-Carrillo C., Reigadas E., Muñoz P, & Escribano P. (2020). Genotyping Reveals High Clonal Diversity and Widespread Genotypes of Candida Causing Candidemia at Distant Geographical Areas. Frontiers in Cellular and Infection Microbiology, 10, 166.

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Genetic Analysis of AIRE Gene Mutations

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In probands, all 14 exons of AIRE gene were amplified by PCR (20 (link), 24 (link)). Bidirectional sequencing was conducted on an ABI3130XL analyser (Applied Biosystems), and sequences were compared with the AIRE mRNA reference sequence (NM_000383.3). In family members, the affected exon was sequenced.
The frequency of the novel AIRE mutation, c.1637G>T, was assessed in 50 healthy subjects by sequencing exon 14. The prevalence of another novel mutation, c.32T>C, was determined in 70 control subjects by PCR-RFLP. A 406-base pair (bp) region of exon 1 was amplified and cut with restriction endonuclease Dde1 (New England Biolabs, Hertfordshire, UK). The wild-type sequence gave bands of 226 and 180 bp, whereas the fragment containing the mutation remained uncut.

Zaidi G., Bhatia V., Sahoo S.K., Sarangi A.N., Bharti N., Zhang L., Yu L., Eriksson D., Bensing S., Kämpe O., Bharani N., Yachha S.K., Bhansali A., Sachan A., Jain V., Shah N., Aggarwal R., Aggarwal A., Srinivasan M., Agarwal S, & Bhatia E. (2017). Autoimmune polyendocrine syndrome type 1 in an Indian cohort: a longitudinal study. Endocrine Connections, 6(5), 289-296.

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