Tetrazolium dye mts cell viability assay kit

Manufactured by Promega

Sourced in United States

The Tetrazolium dye MTS cell viability assay kit is a colorimetric assay for the determination of the number of viable cells in proliferation or cytotoxicity experiments. The assay reagent contains a tetrazolium compound that is bioreduced by metabolically active cells, producing a colored formazan product that is soluble in tissue culture medium.

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Lab products found in correlation

Cytation 5 imaging reader, by Agilent Technologies (2 mentions) Trypsin, by Thermo Fisher Scientific (1 mentions) Trypan blue, by Merck Group (1 mentions)

Close spelling variants of this product

MTS assay (792 citations) MTS kit (98 citations) MTS viability assay (8 citations) MTS dye (8 citations) Cell viability kit (5 citations) MTS cell viability dye (4 citations)

2 protocols using tetrazolium dye mts cell viability assay kit

Evaluating rMSC Viability via MTS Assay

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The viablility of the rMSCs and GpNLuc-rMSCs in growth or osteogenic medium was assessed using tetrazolium dye MTS cell viability assay kit (Promega, USA) following the company’s instructions. At indicated time points, cells were incubated with MTS solution at 37°C for 1 h. The absorbance at 490 nm was measured using the Cytation 5 imaging reader (BioTek, USA).

Wang L., Lee D.J., Han H., Zhao L., Tsukamoto H., Kim Y.I., Musicant A.M., Parag-Sharma K., Hu X., Tseng H.C., Chi J.T., Wang Z., Amelio A.L, & Ko C.C. (2021). Application of bioluminescence resonance energy transfer-based cell tracking approach in bone tissue engineering. Journal of Tissue Engineering, 12, 2041731421995465.

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Cell Viability Assessment Protocols

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For PDHC cytotoxicity assessment, RAW cells were lifted from the material surface using trypsin (Gibco) and then stained with trypan blue (Sigma-Aldrich) for living cell counting, compared to tissue culture plate group. MSC and OB viability was evaluated using tetrazolium dye MTS cell viability assay kit (Promega) following the manufacturer’s instructions. The absorbance at 490 nm was measured using the Cytation 5 imaging reader (BioTek).

Gilliam A.C, & Steitz J.A. (1993). Rare scleroderma autoantibodies to the U11 small nuclear ribonucleoprotein and to the trimethylguanosine cap of U small nuclear RNAs.. Proceedings of the National Academy of Sciences of the United States of America, 90(14), 6781-6785.

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