Protein expression levels of candidate targets of HYD or the herb pair HZ-GC in thyroid tissues obtained from different groups were detected by Western blotting analysis as described in our previous study [41 (link)]. Antibodies against the following proteins were used: Adcy1 (rabbit polyclonal antibody, dilution 1:200, Abcam, Cambridge, UK), Adcy2 (rabbit polyclonal antibody, dilution 1:200, Abcam, Cambridge, UK), Atp1a2 (rabbit monoclonal antibody, dilution 1:500, Abcam, Cambridge, UK), Creb1 (rabbit monoclonal antibody, dilution 1:500, Abcam, Cambridge, UK), Gsr (rabbit monoclonal antibody, dilution 1:2000, Abcam, Cambridge, UK), Hspa5 (rabbit monoclonal antibody, dilution 1:2000, Abcam, Cambridge, UK), Lyd (rabbit polyclonal antibody, dilution 1:1000, Abcam, Cambridge, UK), Pdia4 (rabbit polyclonal antibody, dilution 1:1000, Abcam, Cambridge, UK), Plcb1 (rabbit monoclonal antibody, dilution 1:1000, Abcam, Cambridge, UK), Prkca (rabbit monoclonal antibody, dilution 1:2000, Abcam, Cambridge, UK), Prkcb (rabbit monoclonal antibody, dilution 1:2000, Abcam, Cambridge, UK), Tg (rabbit monoclonal antibody, dilution 1:40000, Abcam, Cambridge, UK), and Tpo (goat polyclonal antibody, dilution 1:500, RD, Minnesota, US). All experiments were performed in triplicate. The mean normalized protein expression ± S.D. was calculated from three independent experiments.
Plcb1
Manufactured by Abcam
Sourced in United Kingdom
Plcb1 is a gene that encodes the protein phospholipase C beta 1. This enzyme is involved in the breakdown of phospholipids, which are important components of cell membranes. Plcb1 plays a role in cellular signaling pathways.
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Lab products found in correlation
2 protocols using plcb1
1
Protein Expression Profiling in Thyroid Tissues
2
Gingival PLCB1 Expression Analysis
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Healthy and diseased gingival biopsies (N = 3/group) were collected, washed in sterile PBS and kept in 1× lysis buffer with protease inhibitor cocktail until further use. Western blot was performed using PLCB1 and glyceraldehyde 3‐phosphate dehydrogenase antibodies (both from Abcam, Cambridge, MA) as previously described (Naqvi et al., 2015 ; Valverde et al., 2020 ).
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