Cd8 bv421 clone rpa t8

Manufactured by BD

CD8-BV421 (clone RPA-T8) is a fluorochrome-conjugated monoclonal antibody that binds to the CD8 antigen expressed on the surface of T lymphocytes. This product can be used for the identification and enumeration of CD8+ T cells in flow cytometry applications.

Automatically generated - may contain errors

Lab products found in correlation

Anti cd3 cd28 microbeads, by Thermo Fisher Scientific (1 mentions) Cd45 apc h7 clone 2d1, by BD (1 mentions) Cd4 fitc clone rpa t4, by BD (1 mentions) Recombinant human il 2, by Thermo Fisher Scientific (1 mentions) Celltrace cfse cell proliferation kit, by Thermo Fisher Scientific (1 mentions) Cd4 percp clone sk3, by BD (1 mentions) Cd45ra fitc clone l48, by BD (1 mentions) Cd3 apc h7 clone sk7, by BD (1 mentions) Facslyric cytometer, by BD (1 mentions) Facsuite clinical software, by BD (1 mentions)

Spelling variants of this product

RPA-T8 (53 citations) CD8 (clone RPA-T8, (17 citations) CD8-BV421 (17 citations) Clone RPA-T8 (14 citations)

2 protocols using cd8 bv421 clone rpa t8

Evaluating T Cell Proliferation by CFSE Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

The proliferation of T cells was quantified by CFSE dilution. Isolated PBMCs were labeled with 2 μM carboxyfluorescein diacetate succinimidyl ester (CFSE; CellTrace CFSE Cell Proliferation Kit, Thermo Fisher Scientific). CFSE-labeled PBMCs (1 × 10⁵/well) were cultured in 96 well plate with or without EVs (4 μg) derived from naive or TSG primed WJ-MSCs, in the presence of anti-CD3/CD28 microbeads (Gibco) and recombinant human IL-2 (30 U/mL, Peprotech). After 6 days of incubation, the cells were stained with human monoclonal antibodies against CD45-APC-H7 (clone 2D1), CD3-PE-Cy5 (clone HIT3α), CD4-FITC (clone RPA-T4), and CD8-BV421 (clone RPA-T8, BD Biosciences) and measured by flow cytometry. All cells were gated on 7AAD negative cells.

Joo H., Oh M.K., Kang J.Y., Park H.S., Chae D.H., Kim J., Lee J.H., Yoo H.M., Choi U., Kim D.K., Lee H., Kim S, & Yu K.R. (2021). Extracellular Vesicles from Thapsigargin-Treated Mesenchymal Stem Cells Ameliorated Experimental Colitis via Enhanced Immunomodulatory Properties. Biomedicines, 9(2), 209.

+ Open protocol

+ Expand

Flow Cytometric Analysis of α7 Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cryopreserved peripheral blood mononuclear cells were thawed, washed, and stained with the following fluorophore-conjugated antibodies to determine the level of  7 expression on CD4 + T cells: CD3-APC-H7 (clone SK7, no. 560176, BD Biosciences), CD4-PerCP (clone SK3, no. 347324, BD Biosciences),  7 -PE (clone FIB504, no. 555945, BD Biosciences), CD45RA-FITC (clone L48, no. 347723, BD Biosciences), and CD8-BV421 (clone RPA-T8, no. 562428, BD Biosciences). Data were acquired by BD FACSLyric cytometer using BD FACSuite clinical software (BD Biosciences) and analyzed using FlowJo version 10.1r5.

Sneller M.C., Clarridge K.E., Seamon C., Shi V., Zorawski M.D., Justement J.S., Blazkova J., Huiting E.D., Proschan M.A., Mora J.R., Shetzline M., Moir S., Lane H.C., Chun T.W, & Fauci A.S. (2019). An open-label phase 1 clinical trial of the anti-α₄β₇ monoclonal antibody vedolizumab in HIV-infected individuals. Science translational medicine, 11(509).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!