Universal mycoplasma detection kit 30 1012

The LY27805 cell line was kindly provided by Bruno Amati’s group [7 (link)] at the European Institute of Oncology–Italian Foundation for Cancer Research (FIRC) Institute of Molecular Oncology (IEO–IFOM, Milan, Italy) campus, expanded and stored according to their instructions. Specifically, LY27805 cells were plated at 2 × 10⁵ cells/mL in B cell medium: 50:50 mixture of DMEM and IMDM (Euroclone, Pero (MI), Italy), 10% FBS (Euroclone, Pero (MI), Italy), 2 mM L-glutamine (Euroclone, Pero (MI), Italy), 1% penicillin/streptomycin (Euroclone, Pero (MI), Italy), 50 μM β-mercaptoethanol (Euroclone, Pero (MI), Italy), and non-essential amino acid (NEEA) (Euroclone, Pero (MI), Italy). Phoenix-Ampho cells were cultured with DMEM (Euclone, Pero (MI), Italy), 10%FBS (Euclone, Pero (MI), Italy), 2 mM L-glutamine (Euclone, Pero (MI), Italy), 1% penicillin/streptomycin (Euclone, Pero (MI), Italy).
Cells were tested and authenticated by the StemElite ID System (Promega, Madison, WI, USA). Cells were tested every six months for Mycoplasma by means of the ATCC Universal Mycoplasma Detection Kit 30-1012, cultured for no more than two weeks, and used for no longer than 15 passages.

The 4T1 BC cell line and the A20 B cell lymphoma cell line were purchased from ATCC, (Manassas, VA, USA), expanded and stored according to the producer’s instructions. Cells were tested and authenticated by the StemElite ID System (Promega, Fitchburg, WI, USA). Cells were tested every six months for Mycoplasma by means of the ATCC Universal Mycoplasma Detection Kit 30–1012, cultured for no more than two weeks and used for no longer than 15 passages.