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1200 system

Manufactured by Phenomenex
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The 1200 system is a liquid chromatography solution from Phenomenex. It is designed to provide reliable and efficient separation of complex samples. The system offers precise control over flow rates, temperature, and other key parameters to ensure consistent and reproducible results. Without making claims about its intended use, the 1200 system is a versatile tool for analytical laboratories.

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Lab products found in correlation

Flow ram radio hplc detector, by Phenomenex (2 mentions) G1315c dad detector, by Agilent Technologies (1 mentions) Gemini nx c18, by Phenomenex (1 mentions) Gemini c18, by Agilent Technologies (1 mentions) 6520 q tof lc ms, by Agilent Technologies (1 mentions)

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1200 HPLC system 1200 LCMS system 1200 Series system

5 protocols using 1200 system

1

Characterization of cyclooctene derivatives

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Materials and general experimental techniques were purchased and conducted, respectively, as per our previous reports.12,13,16 (link) All chemicals were purchased from commercial suppliers and used without purification. 5-Hydroxy-1-cyclooctene (cis-cyclooct-4-enol) was purchased from Carbosynth Limited, UK. Diphenylalanine and 4-amino-2,3,4,6-tetrafluoro benzoic acid were purchased from AK Scientific, USA. Doxorubicin hydrochloride (DOX) was purchased from Lancrix Chemicals, Shanghai, China. All other reagents were purchased from Sigma-Aldrich or AK Scientific. 1H and 13C NMR spectra were recorded on a 400 MHz Varian MR spectrometer. Chemical shifts are reported as δ in parts per million (ppm) and coupling constants are reported as J values in Hz. High resolution electrospray ionization mass spectra (ESI-MS) were recorded on a microTOFQ mass spectrometer. HPLC was performed using an Agilent 1200 system, equipped with a Phenomenex Synergi 4 μm Fusion-RP 80A (150 × 4.6 mm) column, and a photodiode array detector. The applied mobile phases used for kinetic studies were: A, aq. H2O + 0.1% formic acid; and B, MeCN + 0.1% formic acid. Flow speed was 1 mL min−1 and injection volumes were 50 μL. Gradient mobile phase, 80% A/20% B to 100% B in 8 minutes, 2 minutes at 100% MeCN with 0.1% formic acid and returning to starting conditions by 15 minutes.
Dadhwal S., Fairhall J.M., Hook S, & Gamble A.B. (2020). Tetrafluoroaryl azide as an N-terminal capping group for click-to-dissolve diphenylalanine hydrogels. RSC Advances, 10(16), 9234-9244.
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2

Rasfonin Quantification via HPLC-DAD

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Samples were analyzed with an Agilent 1200 system with a reversed phase column Phenomenex Gemini NX C-18 (2 × 150 mm, 5 µm, 110 Å). Injection volume of samples was 20 µL. The flow rate was 0.3 mL/min with a binary gradient of A from 15% (ACN + 0.1% FA) to 95% in the time interval 2–31 min (Solvent B: MQ-H2O + 0.1% FA). Rasfonin was detected with an Agilent G1315C DAD detector at 280 nm. Elution time of rasfonin was at around 21.1 min. For reference measurement and sample spiking, a 25 µg/mL rasfonin standard produced by our laboratory (see above) was used.
Schüller A., Studt-Reinhold L., Berger H., Silvestrini L., Labuda R., Güldener U., Gorfer M., Bacher M., Doppler M., Gasparotto E., Gattesco A., Sulyok M, & Strauss J. (2023). Genome analysis of Cephalotrichum gorgonifer and identification of the biosynthetic pathway for rasfonin, an inhibitor of KRAS dependent cancer. Fungal Biology and Biotechnology, 10, 13.
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3

Radiolabeling of Ybt with Copper-64

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64Cu (t1/2= 12.7 h, β+=17%, β-=39%, EC=43%, Emax=0.656 MeV) was produced by a (p,n) reaction on enriched 64Ni on a CS-15 biomedical cyclotron (Cyclotron Corporation, Berkeley, CA) at Mallinckrodt Institute of Radiology, Washington University School of Medicine and purified on an automated system using standard procedures 58 ,59 . A stock solution of 64Cu in 0.05 M HCl was diluted in 0.1 M ammonium acetate (NH4OAc) buffer solution (pH 7) for radiolabeling purposes. A typical reaction involves using 74 – 111 MBq (2 -3 mCi) of 64Cu added to 20 μL of Ybt (5 nanomoles) and 0.1 M NH4OAc buffer to bring the reaction volume to 100 μL. The reaction mixture was incubated on a mixer with 800 rpm agitation at 37 °C for 1 hour. Labeling efficiency and radiochemical purity were determined using radio-HPLC (Agilent 1200 system with a Flow-RAM radio-HPLC detector) on a C18 column (Kinetex, Phenomenex). The mobile phase consisted of water (A) and acetonitrile (B) with a gradient of 0 – 100 % B over 10 min using a flow rate of 1 mL/min. The retention time of 64Cu(II)-Ybt was 6.1 min and a radiochemical yield of greater than 99% was achieved and used without further purification.
Koh E.I., Robinson A.E., Bandara N., Rogers B.E, & Henderson J.P. (2017). Copper import in Escherichia coli by the yersiniabactin metallophore system. Nature chemical biology, 13(9), 1016-1021.
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4

Radiolabeling of Ybt with Copper-64

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64Cu (t1/2= 12.7 h, β+=17%, β-=39%, EC=43%, Emax=0.656 MeV) was produced by a (p,n) reaction on enriched 64Ni on a CS-15 biomedical cyclotron (Cyclotron Corporation, Berkeley, CA) at Mallinckrodt Institute of Radiology, Washington University School of Medicine and purified on an automated system using standard procedures 58 ,59 . A stock solution of 64Cu in 0.05 M HCl was diluted in 0.1 M ammonium acetate (NH4OAc) buffer solution (pH 7) for radiolabeling purposes. A typical reaction involves using 74 – 111 MBq (2 -3 mCi) of 64Cu added to 20 μL of Ybt (5 nanomoles) and 0.1 M NH4OAc buffer to bring the reaction volume to 100 μL. The reaction mixture was incubated on a mixer with 800 rpm agitation at 37 °C for 1 hour. Labeling efficiency and radiochemical purity were determined using radio-HPLC (Agilent 1200 system with a Flow-RAM radio-HPLC detector) on a C18 column (Kinetex, Phenomenex). The mobile phase consisted of water (A) and acetonitrile (B) with a gradient of 0 – 100 % B over 10 min using a flow rate of 1 mL/min. The retention time of 64Cu(II)-Ybt was 6.1 min and a radiochemical yield of greater than 99% was achieved and used without further purification.
Koh E.I., Robinson A.E., Bandara N., Rogers B.E, & Henderson J.P. (2017). Copper import in Escherichia coli by the yersiniabactin metallophore system. Nature chemical biology, 13(9), 1016-1021.
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5

HPLC Purity Analysis of PAs

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The purity of the PAs after HPLC purification was determined by analytical LC-MS using an Agilent 1200 system equipped with a Phenomenex Gemini C18, 1 × 100 mm column, detector Agilent 6520 Q-TOF LC-MS. Gradient: acetonitrile 5% for 5 min at 50 µL min−1, 5–95% over 25 min at 50 µL min−1 followed by 95% for 5 min at µL min−1. Peaks were detected at 220 nm.
Chin S.M., Synatschke C.V., Liu S., Nap R.J., Sather N.A., Wang Q., Álvarez Z., Edelbrock A.N., Fyrner T., Palmer L.C., Szleifer I., Olvera de la Cruz M, & Stupp S.I. (2018). Covalent-supramolecular hybrid polymers as muscle-inspired anisotropic actuators. Nature Communications, 9, 2395.
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