Hexenal

(-)-Epicatechin (EC), 2´-7´dichlorofluorescein diacetate (DCFH-DA), ampicillin sodium salt, nistatine, agar, yeast extract, fluorodeoxyuridine (FUdR), phosphate-buffered saline (PBS), cholesterol, Bradford reagent, guanidine hydrochloride (GuHCl), 2,4-dinitrophenylhydrazine (DNPH), malondialdehyde, hexanal, hexenal and 4-HNE were purchased from Sigma-Aldrich (Madrid, Spain). Dimethyl sulfoxide (DMSO) was obtained from Panreac (Barcelona, Spain) and trichloroAcetic acid from Fluka Analytical (Madrid, Spain). HPLC grade acetonitrile was from Carlo Erba (Rodano, Italy). Acetic acid was from Merck (Darmstadt, Germany). Fluorescein thiosemicarbazide was from Carbosynth (Berkshire. UK)

The study used n-Alkane (C₇-C₃₀) standards and the available authentic standards, including ethyl acetate (≥99.5%), propyl acetate (≥99.5%), butyl acetate (99.7%), ethyl trans-2-butenoate, ethyl hexanoate (≥99%), ethyl heptanoate (≥99%), ethyl octanoate (≥99%), ethyl 3-hydroxybutyrate (≥ 98%), hexyl hexanoate (≥ 98%), hexanal (≥95%), (Z)-3-hexenal (50% in triacetin), (E)-2-hexenal (≥97%), nonanal (≥99.5%), benzaldehyde (≥99.5%), (E)-2-decenal (≥95%), citral (≥95%), 1-butanol (≥99.4%), 1-hexanol (≥99.9%), (E)-2-hexenol (96%), 1-octen-3-ol (≥98%), 1-heptanol (≥99.5%), 2-ethylhexanol (≥99%), 1-octanol (≥99%), 2-octen-1-ol (97%), (Z)-5-octen-1-ol (≥97%), (E)-5-decen-1-ol (≥97%), phenylethyl alcohol (≥99%), cinnamyl alcohol (≥96%), acetic acid (≥99.7%), hexanoic acid (≥98%), heptanoic acid (≥99%), octanoic acid (99%), nonanoic acid (≥99.5%), limonene (mixture of D- and L-form at ratio of 1:1, ≥95%), linalool (≥99%), citronellol (≥95%), nerol (≥97%), geraniol (≥98.5%), 2-octanone (≥99.5%), acetophenone (≥99.5%), 2-pentylfuran (≥97%), 2-octanol (≥97%), and sodium chloride (NaCl, ≥99%) which were all purchased from Sigma-Aldrich (St. Louis, MO, USA). Geranic acid (sum of isomers, 98%) was purchased from Alfa Aesar Corporation (Tianjin, China). Ultrapure water was prepared using a Milli-Q water purification system (Millipore Corporation, Bedford, MA, USA) with a 0.22 μm filter.