G1888 network headspace autosampler

At least twice during each induction phase, a ~20 microliter blood sample was collected from the saphenous vein of each monkey. Samples were collected 30, 60 or 90 minutes after the start of the session during the 0.5, 1.0 and 1.5 g/kg phases of induction, respectively. These time points capture peak ethanol metabolism and BECs when these doses are orally gavaged in cynomolgus macaques (Green et al., 1999 (link)). Each sample was sealed in an air-tight vial containing 500 microliters of ultrapure HPLC water and 20 microliters of isopropanol (200mg%; internal standard) and stored at −4 ° C until assayed using gas chromatography (Agilent 7890A GC system with G1888 Network Headspace Autosampler Santa Clara, CA supplied with a flame ionization detector and Agilent ChemStation integrator).

Blood samples (20 μl) were collected from the saphenous vein in alert animals while seated in the primate chair. Samples were collected at 30 and 120 min after 2.0 g/kg (i.g.) EtOH was administered when there was no cocaine self-administration sessions conducted (baseline) and on days when cocaine self-administration (0.1 mg/kg/injection) was studied. The order of these two conditions was counterbalanced between monkeys. Each sample was sealed in airtight vials containing 500 μl of distilled water and 20 μl of isopropanol (10%; internal standard) and stored at −4°C until assayed using gas chromatography (Agilent 7890A GC system with G1888 Network Headspace Autosampler Santa Clara, CA) supplied with a flame ionization detector and Agilent ChemStation integrator.

For each monkey, one blood sample (50 microliters) was collected from the saphenous vein 30 minutes after the end of an infusion of 0.5 or 1.0 g/kg ethanol. Blood samples were sealed in airtight vials containing 500 microliters of distilled water and 20 microliters of 10% isopropanol (internal standard) and stored at −4°C until assayed using gas chromatography (Agilent 7890A GC system with G1888 Network Headspace Autosampler Santa Clara, CA) supplied with a flame ionization detector and Agilent ChemStation integrator.