Ultraflex 3 maldi tof tof instrument
The Ultraflex III MALDI-TOF/TOF instrument is a mass spectrometry system designed for the analysis of biomolecules, including proteins, peptides, and oligonucleotides. It utilizes matrix-assisted laser desorption/ionization (MALDI) technology coupled with time-of-flight (TOF) mass analyzers to provide high-performance mass spectrometric analysis.
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10 protocols using ultraflex 3 maldi tof tof instrument
MALDI-TOF/TOF Protein Identification Protocol
MALDI-TOF/TOF Protein Identification
Chemoselective Conjugation of GBS67
Enzymatic Assays for TtAA10 Activity
For MALDI-MS measurements, 1 µl of sample was mixed with an equivalent volume of 10 mg ml−1 2,5-dihydroxybenzoic acid in 50% acetonitrile, 0.1% trifluoroacetic acid on a Bruker SCOUT-MTP 384 target plate. The spotted samples were then dried in air under a lamp before being analysed by mass spectrometry on an Ultraflex III MALDI-TOF/TOF instrument (Bruker), as described by Hemsworth et al. (2014 ▸ ).
MALDI-TOF Mass Spectrometry Analysis of Glycans
Sample permethylation was carried out according to Ciucanu and Kerek55 (link). Spotted samples were analyzed by MS using 2,5-DHB matrix with 0.1% TFA on an AB-Sciex 4700 (for MALDI-TOF) and Ultraflex III MALDI/TOF-TOF instrument (Bruker). Data were collected using a 2-kHz smartbeam-II laser and acquired on reflector mode (mass range 300–3000 Da) for MS analysis and on LIFT-CID for MS/MS analysis using argon as collision gas. FlexControl and FlexAnalysis softwares were used for data acquisition and analysis. On average, about 10,000 shots were used to obtain high-enough resolution. MS/MS fragmentation patterns were named according to Domon and Costello56 (link).
MALDI-TOF Mass Spectra Acquisition
Mass Spectrometry and HPLC Analysis
For the mass spectrometry analysis, an Ultraflex III MALDI-TOF-TOF instrument (Bruker Daltonics GmbH, Bremen, Germany) equipped with a Smartbeam 2 laser with a repetition rate up to 200 Hz (500 laser shots) was used. The obtained mass spectrum was analyzed with Flex-analysis version 4.0 software (Bruker Daltonics GmbH).
The HPLC separation and analysis were performed with Waters® Alliance 2695 (Waters® Corporation, Milford, MA, USA) apparatus equipped with the ultraviolet (UV) detector Waters® 486 (Waters Corporation) and analyzed with Millennium 32 software (Waters Corporation). The reverse phase analytical C8 column (Macherey-Nagel, GmbH & Co, KG, Hoerdt, France) was used for all separations.
MALDI-TOF Quantification of Bcl-2 Protein
Purification and identification of Pdx1 protein
Purification and Characterization of Proteins
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