Bistris native gels

Manufactured by Thermo Fisher Scientific

BisTris native gels are a type of gel electrophoresis system used for the separation and analysis of proteins in their native, non-denatured state. They provide a gentle, non-denaturing environment that helps preserve the structural and functional integrity of proteins during the separation process.

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Lab products found in correlation

Ibright fl1000, by Thermo Fisher Scientific (1 mentions) Sybr gold stain, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Native PAGE BisTris Gels System 4–16%, (2 citations)

2 protocols using bistris native gels

EMSA Analysis of ZBP1 Promoter Binding

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EMSA experiments were performed to examine the binding between unmethylated and methylated promoter regions of ZBP1 labeled with Biotin at the 5'and 3'ends.A mixture of 0.1 pmol of Biotin-tagged DNA fragments, along with 30 pmol of purified CEBPA,was combined in buffer A(20 mM Hepes, pH 7.5,150 mM NaCl,1.5 mM MgCl2,and 2 mM DTT)supplemented with 2 mM ADPCP. This mixture was then left to incubate at room temperature for 10 min before being analyzed using BisTris native gels (Life Technologies).In EMSA experiments that require a specific duration of time,the reactions were incubated at 37° C for the specified period.

Wang A., Zheng W.S., Luo Z., Bai L, & Zhang S. (2024). The innovative checkpoint inhibitors of lung adenocarcinoma, cg09897064 methylation and ZBP1 expression reduction, have implications for macrophage polarization and tumor growth in lung cancer. Journal of Translational Medicine, 22, 173.

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FoxP3 Variants DNA Binding Assay

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0.2 μM of DNA was mixed with the indicated amount of FoxP3 variants or other FoxP proteins in the buffer (20 mM Tris-Hcl pH 7.5, 150 mM NaCl, 1.5 mM MgCl₂, and 2 mM DTT). Proteins and DNAs were incubated for 30 min at 4 °C and analyzed on 3–12% gradient Bis-Tris native gels (Life Technologies) at 4 °C. After staining with Sybr Gold stain (Life Technologies), Sybr Gold fluorescence was recorded using iBright FL1000 (Invitrogen) and analyzed with iBright Analysis Software.

Leng F., Zhang W., Ramirez R.N., Leon J., Zhong Y., Hou L., Yuki K., van der Veeken J., Rudensky A.Y., Benoist C, & Hur S. (2022). The transcription factor FoxP3 can fold into two dimerization states with divergent implications for regulatory T cell function and immune homeostasis. Immunity, 55(8), 1354-1369.e8.

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