Rat anti gr 1

The reagents that have been used in this study were supplied by Sigma‐Aldrich (Saint‐Quentin Fallavier, France). Clobetasol was chosen as it is a potent topical steroid widely used in dermatology for various skin diseases that is able to induce skin atrophy and healing delay.
The following antibodies were used for immunofluorescence staining: rat anti‐mouse F4.80 (1:250; Abcam, Cambridge, UK), rat anti‐Gr‐1 (1:250; eBioscience, Villebon‐sur‐Yvette, France), rat anti‐CD3 (1:250; Biolegend, Paris, France), and rat anti‐mouse CD31 (1:200; BD Biosciences, Le Pont de Claix, France), rabbit anti‐K14 (1:500; Biolegend). The sections were afterwards counterstained with 4,6‐diamidino‐2‐phenylindole 0.3 μg·ml⁻¹ (Sigma‐Aldrich, Saint‐Quentin‐Fallavier, France). Flow cytometry analysis was performed using PE/Cy7‐conjugated rat anti‐F4/80 (1:200, eBioscience), FITC‐conjugated rat anti‐CD31 (1:100, Biolegend), APC‐conjugated rat anti‐CD45 (1:1000; BD Bioscience), APC/Cy7‐conjugated rat anti CD11b (1:100; Biolegend), PE‐conjugated rat anti‐Ly6C (1:100, Biolegend), and FITC‐conjugated rat anti‐CD206 (1:100; Biolegend). The immuno‐related procedures used comply with the recommendations made by the British Journal of Pharmacology (Alexander et al., 2018 (link)).

Rabbit polyclonal antibody against mouse EPRAP was raised by immunization with keyhole limpet hemocyanin–conjugated synthetic peptides corresponding to amino-acid residues 244–260, 330–346, and 629–645 of murine EPRAP. For immunohistochemistry of mouse samples, paraffin-embedded sections were routinely stained with one of the following antibodies: rat anti-F4/80 (Abcam, Cambridge, MA, USA), rat anti–Gr-1 (eBioscience, San Diego, CA, USA), rat anti-B220 (eBioscience), rat anti-CD4 (eBioscience), rat anti-CD8 (Abcam), hamster anti-CD11c (eBioscience), mouse anti-NCAM (Abcam), rabbit anti–phospho-NF-κB p105 (Ser933) (Cell Signaling, Boston, MA, USA), rabbit anti–phospho-MEK1/2 (Ser221) (Cell Signaling), or rabbit anti–phospho-p44/42 MAPK (Thr202/Tyr204) (Cell Signaling), or rabbit anti-EPRAP.
For immunohistochemistry of human samples, paraffin-embedded sections were stained with rat anti-CD68 (Abcam), goat anti-FEM1A (Abcam), and rabbit anti–phospho-NF-κB p105 (Ser933), rabbit anti–phospho-MEK1/2 (Ser221), or rabbit anti–phospho-p44/42 MAPK (Thr202/Tyr204) antibodies. Staining with non-immune rat or rabbit IgG served as a negative control for each experiment.