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Cl 1000s uv crosslinker

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The CL-1000S UV crosslinker is a laboratory instrument used for exposure of samples to ultraviolet (UV) light. It provides controlled and reproducible UV irradiation for various applications.

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Lab products found in correlation

Lipofectamine rnaimax transfection reagent, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

CL-1000 (53 citations) CL-1000 UV Crosslinker (25 citations) CL-1000 crosslinker (24 citations) UV crosslinker (9 citations)

4 protocols using cl 1000s uv crosslinker

1

UV-C Radiation Exposure Assay

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Mammalian cells (HCT116 or MEFs) were plated 24 hours before
irradiation. Immediately prior to irradiation, media was aspirated and the
cells were briefly washed with dPBS. With lids and dPBS removed, culture
plates containing the cells were exposed directly to UV-C radiation in a
254nm UV oven (CL-1000S UV crosslinker, UVP), then media was replenished.
All experiments here involved two equal doses of UV radiation, 24 hours
apart. Reported radiation doses are the total combined dose from two
treatments. Unless otherwise indicated, 48 hours following the terminal
dose, cells were trypsinized and resuspended in serum-free media for flow
cytometry analysis or sorting.
Birnbaum M.D., Nemzow L., Kumar A., Gong F, & Zhang F. (2018). A Rapid and Precise Mutation-Activated Fluorescence Reporter for Analyzing Acute Mutagenesis Frequency. Cell chemical biology, 25(8), 1038-1049.e5.
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2

Peptide-Mediated Gold Nanoparticle Synthesis

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Two milligrams
of purified peptide was dissolved in Milli-Q water under vortex until
complete dissolution. This peptide solution was then homogeneously
mixed with 0.18, 0.36, and 0.72 mM HAuCl4 solution. The
sample mixtures were vortexed for 1 min, and the samples were exposed
to UV light using a UVP CL-1000s UV Crosslinker at 254 nm wavelength
with an intensity of 2.4 W/cm2 for 30 min. The stability
of the GPNP suspension was observed for up to 14 days.
Abbas M., Susapto H.H, & Hauser C.A. (2022). Synthesis and Organization of Gold-Peptide Nanoparticles for Catalytic Activities. ACS Omega, 7(2), 2082-2090.
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3

UV-C Irradiation of Cells

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UV irradiations of yeast cells or mammalian cells was performed in a 254 nm UV-C irradiation oven (CL-1000S UV crosslinker from UVP, Upland, CA, USA) in a dark room.
Kumar A., Birnbaum M.D., Patel D.M., Morgan W.M., Singh J., Barrientos A, & Zhang F. (2016). Posttranslational arginylation enzyme Ate1 affects DNA mutagenesis by regulating stress response. Cell Death & Disease, 7(9), e2378-.
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4

UV-induced p53 Knockdown in HCT116 Cells

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HCT116 cells were cultured in six-well tissue culture plates (~1 × 10 5 cells per well) or 10-cm dishes (~2 × 10 6 cells per dish). The cells were transfected with 25 nM siRNAs targeting p53 or 25 nM siCONTROL NON-TARGETING siRNA using Lipofectamine RNAiMAX Transfection Reagent (Invitrogen). Cells were harvested at 48 h after transfection. Then the cells were treated with 20 J of UV (254-nm peak)/m 2 by CL-1000S UV Crosslinker (UVP, Upland, CA). After 1-h incubation, the cells were harvested.
Anwar D., Takahashi H., Watanabe M., Suzuki M., Fukuda S, & Hatakeyama S. (2016). p53 represses the transcription of snRNA genes by preventing the formation of little elongation complex. Biochimica et biophysica acta, 1859(8).
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