F12k media

H. pylori strains 26695 and ΔcagV (hp0530) mutant have been described^{36 (link)} and were cultivated on Columbia agar base (BD) containing 10% (v/v) defibrinated horse blood (Wisent Inc.), vancomycin (10 μg/ml) and amphotericin B (10 μg/ml). Chloramphenicol (34 μg/ml) was added in case of the ΔcagV strain to select for the cam gene cassette used to disrupt the gene. For liquid culture, brain heart infusion (BHI) media (Oxoid) were supplemented with 8% fetal bovine serum (FBS) and appropriate antibiotics. Bacteria were cultivated at 37 °C, under microaerophilic conditions (5% oxygen, 10% CO₂). AGS cells were grown at 37 °C in F12K media (Wisent Inc.) with 10% (v/v) FBS (Wisent Inc.) in a 5% CO₂ containing atmosphere.

HeLa and HFF-1 cells obtained from the American Type Culture Collection (ATCC) were grown in DMEM (Wisent), while A549 cells were grown in F12K media (Wisent) and HCT 116 (p53-/-) cells were grown in McCoy’s media (Wisent) in humidified incubators at 37 °C with 5% CO₂. All media were supplemented with 10% fetal bovine serum (FBS; Thermo Scientific), 2 mM l-glutamine (Wisent), 100 U penicillin (Wisent), and 0.1 mg/mL streptomycin (Wisent).
C87 and C75 were stored at − 20 °C as 1 mM stocks in dimethyl sulfoxide (DMSO). Working stocks of 100 μM C75 or C87 were made in DMSO:H₂O (9:1). Colchicine (Sigma) was dissolved in ethanol as a 1 M stock and diluted to 10 μM before use. Nocodazole was dissolved in DMSO as a 1 mg/mL stock (Sigma). Paclitaxel (Bioshop) was dissolved in DMSO as a 10 mM stock and diluted to a range of smaller concentrations before use. Cells grown as monolayer cultures or as spheroids were treated with C75, C87, Colchicine, or paclitaxel as indicated, and the final concentrations of DMSO or ethanol were kept below 0.5%.