MatriGel (Corning, USA) was mixed with serum-free medium at 1:9 proportion and placed above Insert cell culture dish (Millipore, Germany) the night before and placed in a 37℃ incubator, then 1 × 105 cells in serum-free medium were placed above the dish with or without MatriGel for 24 and 48 h, the dishes were fixed with 4% paraformaldehyde and stained with Crystal Violet solution (Biosharp, China), the images were acquired using the Inverted microscope (Olympus, Japan).
Matrigel
Manufactured by Biosharp
Sourced in China
MatriGel is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma. It is a complex mixture of extracellular matrix proteins, growth factors, and other components. MatriGel provides a substrate for cell attachment, differentiation, and three-dimensional culture.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using matrigel
1
Cell Invasion Analysis on MatriGel
2
Endothelial Cell Tube Formation Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were cultured in 3 groups, as mentioned above. Each group consisted of three replicate wells, totaling nine wells. HUVEC was seeded in 96-well plates at a density of 2–3 × 104 and was precooled with 50 μL Matrigel (Biosharp, China) per well (the matrix gel was thawed at 4 °C and mixed uniform by using cooled pipette tips) and incubated for 1h at a cell incubator. Then added 50 μL culture medium to each well and set the plates at 37 °C for 18 h. All operations need to be careful to avoid bubbles. Finally, observe the plates after staining endothelial tubes with Calcein AM dye. Five visual fields were randomly chosen under a 100 × fluorescent microscope to observe and count the endothelial cells.
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