Rabbit anti atg5

Primary antibodies used were: mouse anti-BrdU (G3G4; DSHB), mouse anti-GFAP (Thermo Fisher Scientific), rhodamine-conjugated mouse anti-BrdU (Millipore), mouse anti-nestin (Rat-401), mouse anti-NeuN (Millipore), mouse anti–Nqo-1 (Santa Cruz Biotechnology), mouse anti-p62 (Abcam), mouse anti ubiquitin (Santa Cruz Biotechnology), mouse antivinculin (Sigma-Aldrich), rabbit anti-Atg5 (Novus Biologicals), rabbit anti-Atg16L1 (Abgent), rabbit anti-GFAP (Dako), rabbit anti-Ki67 (Spring Bioscience), rabbit anti-LC3 (Cell Signaling), rabbit anti-NBR1 (Cell Signaling), rabbit anti-Nrf2 (Abcam), rabbit anti-p62 (Enzo), rabbit anti-Sox2 (Millipore), rabbit anti-TAX1BP1 (Abgent), rabbit anti-Fip200 (ProteinTech), rabbit anti-olig2 (Millipore), rat anti-Ki67 (BioLegend), and guinea pig antidoublecortin (anti-DCX; EMD Millipore). Secondary antibodies were goat anti–rabbit IgG-FITC, goat anti–rabbit IgG–Texas red, goat anti–mouse IgG-FITC, goat anti–mouse IgG–Texas red, goat anti–mouse IgG-HRP, and goat anti–rabbit IgG-HRP (Jackson Immunology).
DHE and EUK-8 were purchased from Sigma-Aldrich. EUK-134 was purchased from Cayman Chemical. DCFDA, MitoTracker Red, and MitoTracker Green were purchased from Invitrogen.

Protein analysis was performed by exponential Bio-Rad Mini-PROTEAN® TGX Gels (Bio-Rad, Hercules, CA, USA), followed by a semi-dry transfer (Bio-Rad) on nylon membrane Hybond™-P (GE Healthcare Amersham, Thermo Fisher Scientific). Protein expression was detected by incubation with different primary polyclonal antibodies: rabbit anti-peroxiredoxin 6™ Picoband (Boster, Pleasanton, CA, USA), rabbit anti-p62/SQTM1 and rabbit anti-atg5 (Novus Biologicals, Centennials, CO, USA), followed by incubation with polyclonal goat anti-rabbit immunoglobulin/HRP (Dako Agilent, Santa Clara, CA, USA), followed by an enhanced chemiluminescent substrate ECL (Pierce™ ECL Western Blotting Substrate, Thermo Fisher). Bands were visualized using an AutoChemi Imaging System (BioImaging Systems, UPV Inc., Upland, CA, USA).