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Anti mouse hrp dab cell tissue staining kit

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The Anti-Mouse HRP-DAB Cell & Tissue Staining Kit is a laboratory tool designed for the detection and visualization of mouse antigens in cell and tissue samples. It utilizes a horseradish peroxidase (HRP)-based detection system and 3,3'-Diaminobenzidine (DAB) as the chromogenic substrate to produce a brown staining reaction.

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Lab products found in correlation

Ab64883, by Abcam (1 mentions) Dspp sc 73632, by Santa Cruz Biotechnology (1 mentions) Ab32572, by Abcam (1 mentions) Normal mouse igg, by Abcam (1 mentions) Mouse anti human thrombin antibody, by Abcam (1 mentions) Axio observer, by Zeiss (1 mentions)

Close spelling variants of this product

Anti-mouse/rabbit HRP-DAB Cell & Tissue Staining Kit HRP‐DAB Cell & Tissue Staining Kit

5 protocols using anti mouse hrp dab cell tissue staining kit

1

Evaluating Cartilage Regeneration via Immunohistochemistry

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Eight weeks later, animals were euthanatized by CO2 followed by cervical dislocation, the transplants were collected and fixed with 4% paraformaldehyde. After fixation, samples were demineralized in 0.5 M EDTA (pH 7.4) for 1 week and then dehydrated. Paraffin sections (5 mm) were used for hematoxylin-eosin (HE), Masson’s trichrome and immunohistochemical staining. We used Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (R & D Systems) in the immunohistochemical staining. After deparaffinizing, heat-retrieving and blocking, sections were incubated with the primary antibodies (Brdu, ab270260, Abcam; Col11A1, ab64883, Abcam) overnight at 4 °C, then incubated with HRP conjugated secondary antibodies. Finally, images were developed with DAB. We stained three tissue sections per sample. The ROIs were calcified region, and we randomly selected three fields (400X). The BrdU and Col1 were analyzed by their integrated optical density (IOD) value via image pro plus 6.0 software. When we calculated the IOD, we eliminated the color “blue” interference which stands for the nucleus.
Jin Y., Sun X., Pei F., Zhao Z, & Mao J. (2020). Wnt16 signaling promotes osteoblast differentiation of periosteal derived cells in vitro and in vivo. PeerJ, 8, e10374.
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2

Tissue Histology and IHC Analysis

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5 µm-thick tissue sections were generated from Formalin-Fixed Paraffin Embedded (FFPE) tumor samples and stained with Hematoxylin and Eosin (H&E) for standard histology observations. ImmunoHistoChemistry (IHC) stainings were also performed using an anti-Ki67, anti-Cleaved Caspase-3 or anti-AXL antibodies with the avidin-biotin-peroxidase techniques (Anti-Mouse HRP-DAB Cell & Tissue Staining Kit, R&D Systems). Slides were counterstained with Hematoxylin.
Quirico L., Orso F., Esposito C.L., Bertone S., Coppo R., Conti L., Catuogno S., Cavallo F., de Franciscis V, & Taverna D. (2020). Axl-148b chimeric aptamers inhibit breast cancer and melanoma progression. International Journal of Biological Sciences, 16(7), 1238-1251.
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3

Immunohistochemical Evaluation of Aurora A

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TMAs were constructed by selecting viable and representative regions enriched for tumour cells from formalin‐fixed and paraffin‐embedded tumour tissues as previously described 29, 31. A total of 14 TMA slides representing three core samples from each case were stained by IHC with the Aurora A [35C1] antibody (GeneTex, cat. GTX13824) at a dilution of 1:50. Heat‐induced antigen retrieval was achieved in a 10 mm citrate buffer pH 6 for 10 min in an autoclave at 120°C following overnight incubation with the Aurora A antibody at 4°C in a humid chamber. Anti‐mouse HRP‐DAB cell & tissue staining kit (R&D systems; cat. CTS002) was used for antibody detection following the manufacturer's recommendations. Sections were then counterstained with haematoxylin eosin. The sections were scored positive or negative according to Aurora A nuclear staining. Positive cytoplasmic staining without nuclear staining was defined as negative. The scoring was done by subjective assessment. All sections were scored by the same two individuals in a blinded manner.
Aradottir M., Reynisdottir S.T., Stefansson O.A., Jonasson J.G., Sverrisdottir A., Tryggvadottir L., Eyfjord J.E, & Bodvarsdottir S.K. (2014). Aurora A is a prognostic marker for breast cancer arising in BRCA2 mutation carriers. The Journal of Pathology: Clinical Research, 1(1), 33-40.
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4

Immunohistochemical Analysis of DSPP and CTNNB1

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Tissue sections were stained with hematoxylin-eosin. Immunohistochemical staining was done using Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (R&D Systems). Briefly, sections were deparaffinized, heat-retrieved, blocked and thereafter incubated with the primary antibodies (DSPP, sc-73632, 1:200, Santa Cruz Biotechnology) overnight at 4°C, followed by washing and incubation with HRP conjugated secondary antibodies. Images were developed with DAB finally. For staining of CTNNB1 (β-catenin) in cells, control and Lhx8 overexpressed cells were grown on the coverslip at the 70–80% confluence. Following blocking of nonspecific binding sites, cells were incubated with anti-CTNNB1 antibody (ab32572, 1:200, Abcam) overnight at 4°C. The experiments were repeated at least three times.
Zhou C., Yang G., Chen M., Wang C., He L., Xiang L., Chen D., Ling J, & Mao J.J. (2015). Lhx8 Mediated Wnt and TGFβ Pathways in Tooth Development and Regeneration. Biomaterials, 63, 35-46.
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5

Localization of Basal Prothrombin in Fetal Membranes

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To determine basal prothrombin protein localization in fetal membranes, freshly harvested, full-thickness fetal membranes without U. parvum infection were fixed in 10% formalin for IHC. Paraffin sections were de-waxed and rehydrated. After heat-induced antigen retrieval, the sections were stained using the anti-mouse HRP-DAB Cell & Tissue Staining Kit following manufacturer’s instruction (R&D Systems). Mouse anti-human thrombin antibody (Abcam) was used at a 1:500 dilution in PBS with 1% BSA and 5% goat serum. Placenta tissue was used as positive control. For each batch of staining, one slide was incubated with normal mouse IgG (Abcam) instead of the primary antibody as a negative control. Images were taken using a Zeiss Axio Observer (20X). Three rounds of IHC staining were performed using fetal membrane tissues collected from three subjects.
Feng L., Allen T.K., Marinello W.P, & Murtha A.P. (2018). Infection-Induced Thrombin Production: A Potential Novel Mechanism for Preterm Premature Rupture of Membranes (PPROM). American journal of obstetrics and gynecology, 219(1), 101.e1-101.e12.
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