Sudhl 5

The 16 DLBCL cell lines (DOHH2, HT, OCI-LY19, DB, OCI-LY1, SUDHL-4, SUDHL-5, SUDHL-10, NUDHL-1, OCI-LY7, WSU-DLCL2, SUDHL-6, NUDUL-1, U2932, OCI-LY3, and RI-1) were purchased from the American Type Culture Collection or from DSMZ (Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Germany). They were grown in RPMI-1640 Glutamax medium (Gibco, Invitrogen, Cergy Pontoise, France), supplemented with 10% fetal bovine serum (FBS) (PAA laboratory GmbH, Pasching, Austria) (U2932, SUDHL-4, HT, DOHH2, SUDHL-10, RI-1, and WSU-DLCL2 cells), 20% FBS (OCI-LY3, DB, SUDHL-5, NUDHL-1, and SUDHL-6 cells), or 15% FBS (NUDUL-1 cells). OCI-LY1, and OCI-LY7 cells were cultured in IMDM Glutamax (Gibco, Invitrogen, Cergy Pontoise, France), supplemented with 20% FBS, and OCI-LY19 cells in MEM alpha modified Glutamax (Gibco, Invitrogen, Cergy Pontoise, France) with 20% FBS. Cultures were maintained at 37 °C in a humidified atmosphere with 5% CO₂. Contamination by Mycoplasma species was regularly monitored.

Human DLBCL cell lines (U2932, HT, SU-DHL-5, and RC-K8) were purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA) and the Bioresource Collection and Research Center (BCRC, Hsinchu, Taiwan). The DLBCL cell lines were cultured at 37 °C in a humidified 5% CO₂ incubator in RPMI 1640 (HCC38) supplemented with 10% fetal bovine serum ((FBS) Merck, Darmstadt, Germany), 2% penicillin-streptomycin ((P/S) Gibco Thermo Fisher Scientific, Waltham, MA, USA), 10 mM HEPES (Gibco Thermo Fisher Scientific), 2 mM L-glutamine (Gibco Thermo Fisher Scientific), 2 g/L D-glucose (Sigma Aldrich, St Louis, MO, USA), and 1 mM Sodium Pyruvate (Gibco Termo Fisher Scientific).