Igf1rfl fl

All experimental procedures were performed in compliance with animal protocols approved by the IACUC at Boston Children’s Hospital and Washington University in St. Louis. Mice aged 3–5 weeks at the start of experiments were used throughout. Male and female mice were used in this study at ratios dependent on litters available and with equal distributions across experiments conducted extemporaneously. C57BL6/J, Vglut2-ires-Cre (028863; Jackson Labs), Vgat-IRES-Cre (016962; Jackson Labs), IFT88fl/fl (022409; Jackson Labs) and IGF1Rfl/fl (mouse lines were obtained from Jackson Laboratories. The connexin 57cre (Cx57-Cre) mouse line was described in (Hirano et al., 2016 ).

C57BL/6J, Igf1r^fl/fl, and Igf1^fl/fl mice were obtained from Jackson Laboratories. CCSP-_rtTA/tetO-Cre were kindly provided by Dr. Jeffrey Whitsett at Cincinnati Children’s Hospital^{37 (link)}. To generate IGF-1R deletion in Club cells, we crossed CCSP-_rtTA/tetO-Cre mice to Igf1r^{fl/fl36 (link)}. To achieve deletion, mice were given doxycycline (1mg/mL) in drinking water containing 0.4% sucrose for at least 7 days prior to beginning of allergen administration, unless otherwise noted. We also crossed Igf1^fl/fl mice^{33 (link),34 (link)} to LysM-Cre mice to conditionally delete Igf1 in the myeloid lineage. We have previously reported on the generation of CCSP-Cre/YFP mice^{4 (link)}. For all in vivo experiments, except generation of apoptotic thymocytes, mice between the ages of 8 and 12 weeks were used. No blinding was performed for in vivo experiments. Mice were allocated to experimental groups based on genotype and age-matching. Female and male mice were used for all experiments, except for in vivo engulfment assays in which only male mice were used. Sample size was selected based on mice availability and power statistics. All animal procedures were performed according to the protocols provided by the Institutional Animal Care and Use Committee (IACUC) of the University of Virginia.