α parvin

PLC-γ1, eIF4H, α-Parvin and ß-actin were obtained from Cell Signaling Technology (Danvers, MA, USA).

Protein samples from human melanoma cells were subjected to SDS-PAGE and Western Blot analysis. Briefly, cells were lysed using RIPA B buffer containing proteases and phosphatases inhibitors (ROCHE) to extract the proteins. Protein concentration was determined using Bio-Rad protein assay (Bio-Rad) and the Epoch spectrophotometer (BioTek). SDS-poly-acrylamide gels were used to separate the proteins by molecular weight. Western blots were performed to detect the following proteins of interest: ADAR1 (Sigma, Cat. No. SAB4200541), α-tubulin (Sigma, Cat. T-5168), actin (Sigma, Cat. A-20665168), c-Jun Ser 63/73 (Santa Cruz, Cat. No. sc-16312), α-parvin (Cell Signaling, Cat. No. 4026), AKT Ser 473 (Cell Signaling, Cat. No. 9271) and MMP2 (Cell Signaling, Cat. No. 4022). Supplementary Fig. 6 shows the full blots.