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Aldh2 activity assay kit

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The ALDH2 activity assay kit is a quantitative colorimetric assay designed to measure the activity of the aldehyde dehydrogenase 2 (ALDH2) enzyme in biological samples. The kit provides a simple and reliable method to determine ALDH2 activity.

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Lab products found in correlation

Sodium hydrosulfide nahs, by Merck Group (1 mentions) Anti aldh2 antibody, by Abcam (1 mentions) Alcohol dehydrogenase activity colorimetric assay kit, by Abcam (1 mentions)

7 protocols using aldh2 activity assay kit

1

Quantifying Hepatic ALDH2 Activity

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The activity of ALDH2 was analyzed using a colorimetric Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit (ab115348; Abcam, Cambridge, UK) according to the manufacturer’s protocol using 200 μg of total protein from liver homogenate.
Hsu L.A., Tsai F.C., Yeh Y.H., Chang C.J., Kuo C.T., Chen W.J., Tsai H.Y, & Chang G.J. (2020). Aldehyde Dehydrogenase 2 Ameliorates Chronic Alcohol Consumption-Induced Atrial Fibrillation through Detoxification of 4-HNE. International Journal of Molecular Sciences, 21(18), 6678.
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2

Assessing ALDH2 Expression and Activity

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Human ALDH2 mRNA levels in the liver were assessed by TaqMan RT‐qPCR using FAM dye–labeled hALDH2‐specific primer‐probe sets (Assay ID; Hs01007998_ml) and murine 18 S specific primer‐probe sets (Life Technologies, Waltham, MA USA) and compared with a standard curve derived from the pAAV‐CAG‐hALDH2‐HA expression plasmid. For male mice, n = 6 mice were processed for all groups; for female mice, AAVrh.10hALDH2 treated (n = 6), AAVrh.10control treated (n = 5), PBS treated (n = 4). The enzymatic activity of ALDH2 in liver was analyzed using the colorimetric mitochondrial aldehyde dehydrogenase (ALDH2) activity assay kit (Abcam; ab115348) according to the manufacturer's protocol using 200 μg of total protein from liver homogenate. For male mice, ethanol plus AAVrh.10hALDH2 (n = 6), ethanol plus AAVrh.10control (n = 6), ethanol plus PBS (n = 6), and water plus PBS (n = 5); for female mice, ethanol plus AAVrh.10hALDH2 (n = 6), ethanol plus AAVrh.10control (n = 5), ethanol plus PBS (n = 4), and water plus PBS (n = 6).
Camilleri A.E., Cung M., Hart F.M., Pagovich O.E., Crystal R.G., Greenblatt M.B, & Stiles K.M. (2023). Gene Therapy to Treat Osteopenia Associated With Chronic Ethanol Consumption and Aldehyde Dehydrogenase 2 Deficiency. JBMR Plus, 7(4), e10723.
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3

Hepatic ALDH2 and Lipid Peroxidation

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Hepatic ALDH2 activity changes after alcoholic liver injury and/or stem cell transplantation were measured using an ALDH2 activity assay kit (Abcam). Levels of the end-product of lipid peroxidation (MDA) in the liver tissue samples were determined using a Bioxytech LPO-586™ kit (Oxis Research, Portland, OR, USA). The reaction products were measured spectrophotometrically at 586 nm.
Li M., Lv Y., Chen F., Wang X., Zhu J., Li H, & Xiao J. (2018). Co-stimulation of LPAR1 and S1PR1/3 increases the transplantation efficacy of human mesenchymal stem cells in drug-induced and alcoholic liver diseases. Stem Cell Research & Therapy, 9, 161.
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4

Hcy-Induced ALDH2 Modulation and Oxidative Stress

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Sodium hydrosulfide (NaHS, a donor of H2S) and Hcy were purchased from Sigma Chemical. Anti-ALDH2 antibody was purchased from Abcam Technology. ALDH2 activity assay kit was obtained from Abcam. MDA enzyme linked immunosorbent assay (ELISA) kit was purchased from Uscn Life Science. 4-HNE and GSH ELISA kits were obtained from Bio-Swamp Life Science.
Li M., Zhang P., Wei H.J., Li M.H., Zou W., Li X., Gu H.F, & Tang X.Q. (2016). Hydrogen Sulfide Ameliorates Homocysteine-Induced Cognitive Dysfunction by Inhibition of Reactive Aldehydes Involving Upregulation of ALDH2. International Journal of Neuropsychopharmacology, 20(4), 305-315.
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5

Colorimetric ALDH2 Activity Assay

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The enzymatic activity of ALDH2 was analyzed using the colorimetric mitochondrial aldehyde dehydrogenase (ALDH2) Activity Assay Kit (Abcam, ab115348, Cambridge, MA) according to the manufacturer’s recommendations using 600 μg of total protein from cell samples.
Wang X., Wang J., Chen Y., Qian X., Luo S., Wang X., Ma C, & Ge W. (2024). The aldehyde dehydrogenase 2 rs671 variant enhances amyloid β pathology. Nature Communications, 15, 2594.
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6

Quantifying ALDH2 Enzyme Activity

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The ALDH2 activity was assessed using the mitochondrial aldehyde dehydrogenase (ALDH2) activity assay kit (ab115348, Abcam Co., Cambridge, UK). Briefly, the activity is determined by following the production of NADH in the following ALDH2 catalyzed reaction: acetaldehyde + NAD+ → acid + NADH, we determined the activity of ALDH2 by measuring absorbance of acid at 450 nm. All reagents were provided and we conducted the experiment according to the manufacturer's protocol.
Gu X., Fang T., Kang P., Hu J., Yu Y., Li Z., Cheng X, & Gao Q. (2017). Effect of ALDH2 on High Glucose-Induced Cardiac Fibroblast Oxidative Stress, Apoptosis, and Fibrosis. Oxidative Medicine and Cellular Longevity, 2017, 9257967.
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7

Intrahepatic Alcohol-Metabolizing Enzyme Assays

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Intrahepatic alcohol dehydrogenase 1 (ADH1) and aldehyde dehydrogenase 2 (ALDH2) activities were measured by using Alcohol Dehydrogenase Activity Colorimetric Assay Kit (BioVision, Milpitas, CA, United States) and ALDH2 activity assay kit (Abcam), respectively, according to the manufacturer’s instructions. Intrahepatic CYP2E1 activity was determined by measuring the hydroxylation of p-nitrophenol in whole liver extract as described[30 (link)].
Fujimoto Y., Kaji K., Nishimura N., Enomoto M., Murata K., Takeda S., Takaya H., Kawaratani H., Moriya K., Namisaki T., Akahane T, & Yoshiji H. (2021). Dual therapy with zinc acetate and rifaximin prevents from ethanol-induced liver fibrosis by maintaining intestinal barrier integrity. World Journal of Gastroenterology, 27(48), 8323-8342.
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