Anti cyclin a2

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Anti-cyclin A2 is a laboratory reagent used for the detection and quantification of cyclin A2 in biological samples. Cyclin A2 is a cell cycle regulatory protein that plays a critical role in the progression of cells through the cell cycle. The Anti-cyclin A2 reagent can be used in various applications, such as Western blotting, immunoprecipitation, and immunohistochemistry, to study the expression and localization of cyclin A2 in cells and tissues.

Automatically generated - may contain errors

Lab products found in correlation

Anti cyclin b1, by Santa Cruz Biotechnology (3 mentions) Anti β actin, by Merck Group (2 mentions) Anti cleaved caspase 3, by Cell Signaling Technology (2 mentions) Anti bax antibodies, by BD (2 mentions) Anti s100a1, by Abcam (2 mentions) Anti cleaved poly adp ribose polymerase 1 parp1 asp214 d64e10, by Cell Signaling Technology (2 mentions) Adriamycin adr, by Merck Group (2 mentions) Nutlin 3a, by Merck Group (2 mentions) Anti ki67, by Agilent Technologies (2 mentions) Anti p27kip1, by BD (2 mentions) Anti β actin antibody, by Merck Group (2 mentions) Anti flag m2, by Merck Group (2 mentions) Anti mdm2, by Abcam (2 mentions) Anti p53, by Agilent Technologies (2 mentions) Anti bcl2, by Agilent Technologies (2 mentions) Pvdf membrane, by Bio-Rad (1 mentions) Anti flag, by Merck Group (1 mentions) Anti cdc25c, by Santa Cruz Biotechnology (1 mentions) Anti prb, by Santa Cruz Biotechnology (1 mentions) Anti cyclin d3, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

Cyclin A2 (17 citations)

7 protocols using anti cyclin a2

Western Blot Analysis of Cell Cycle Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were lysed by sonication in RIPA buffer and the protein concentration was determined using a Nanodrop. Equal protein amounts were resolved by SDS-PAGE (10%). Proteins were transferred to PVDF membranes (Bio-Rad) by electroblotting. The membranes were blocked with 5% nonfat dry milk in TBST buffer and analyzed by Western analysis with anti-Flag (Sigma), anti-Cdc25A (Thermo Scientific), and/or anti-β-actin (Sigma). Anti-Cdc25B, anti-Cdc25C, anti-cyclin A2, anti-cyclin E1, anti-cyclin D1, anti-cyclin D3, and anti-pRB were purchased from Santa Cruz Biotechnology.

Lozada E.M., Andrysik Z., Yin M., Redilla N., Rice K, & Stambrook P.J. (2016). Acetylation and deacetylation of Cdc25A constitutes a novel mechanism for modulating Cdc25A functions with implications for cancer. Oncotarget, 7(15), 20425-20439.

+ Open protocol

+ Expand

Tissue Sectioning and Immunostaining for Microscopy

Check if the same lab product or an alternative is used in the 5 most similar protocols

OCT-embedded tissue blocks were mounted onto an HM550 cryostat for sectioning. For confocal microscopy applications, the tissues were cryosectioned at 12 to 15 µm and loaded onto Superfrost™ plus slides (Fisher) and immunostained using anti-Cyclin A2 (Santa Cruz Biotechnology, Santa Cruz, CA, SC-596) and anti-phospho-Histone H3 (Cell Signaling, Danvers, MA, 9706S); secondary antibodies were purchased from Molecular Probes (Billerica, MA), and nuclei were counterstained with DAPI (Sigma, St. Louis, MO). An LSM700 Zeiss Confocal Microscope was used and images were captured as .czi files using Zen™ software. Post-capturing, images were opened in FIJI, colors were separated, and files saved as TIFF images. Collages were generated using Adobe Photoshop CS6 and Adobe Illustrator CS6.

Chang J.C., Leung M., Gokozan H.N., Gygli P.E., Catacutan F.P., Czeisler C, & Otero J.J. (2015). Mitotic Events in Cerebellar Granule Progenitor Cells that Expand Cerebellar Surface Area Are Critical for Normal Cerebellar Cortical Lamination in Mice. Journal of neuropathology and experimental neurology, 74(3), 261-272.

+ Open protocol

+ Expand

Protein Expression Analysis by SDS-PAGE and Western Blot

Check if the same lab product or an alternative is used in the 5 most similar protocols

Standard sodium dodecyl sulphate-polyacrylamide gel electrophoresis and Western blot analysis was performed. Primary antibodies used were anti-cyclin A1 (BD Biosciences, San Diego, CA, USA), anti-cyclin A2 (Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-c-MYC (Roche Diagnostics, Indianapolis, IN, USA) and anti-beta-actin (Sigma-Aldrich, Natick, MA, USA). Peroxidase-based pico-chemiluminescence kit (Thermo Fisher Scientific, MA, USA) was used to reveal antibody signal.

Huang K.C., Yang J., Ng M.C., Ng S.K., Welch W.R., Muto M.G., Berkowitz R.S, & Ng S.W. (2016). CyclinA1 Expression and Paclitaxel Resistance in Human Ovarian Cancer Cells. European journal of cancer (Oxford, England : 1990), 67, 152-163.

+ Open protocol

+ Expand

Comprehensive Immunoblotting Antibody Panel

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following commercially available primary antibodies were used for immunoblotting: anti-IRS2 (Cell Signaling Technologies, 4502) 1:750; anti-Cdh1/Fzr1 (Sigma Aldrich, CC43) 1:500; anti-anillin (a gift from Christine Field (21 (link))) 1:1000; anti-Aurora B (Bethyl, A300-431) 1:1000; anti-Eg5 (Cell Signaling Technologies, 4203) 1:1000; anti-Top2A (Cell Signaling Technologies, 12286) 1:1000; anti-TK1 (Cell Signaling Technologies, 8960) 1:1000; anti-Mps1 (Abcam, ab11108), 1:1000); anti-APC3 (BD Transduction Laboratories, 610455) 1:500; anti-cyclin B1 (Santa Cruz Biotechnology, sc-752) 1:500; anti-Cdc20 (Santa Cruz Biotechnology, sc-8358) 1:500; anti-c-Myc (9E10, Santa Cruz Biotechnology, sc-40) 1:1000; anti-HA-peroxidase (Sigma Aldrich), 1:1500; anti-cyclin A2 (Santa Cruz Biotechnology, sc-596) 1:500; anti-IRS1 (Cell Signaling Technologies, 2382) 1:750; anti-MyoD1 (Cell Signaling Technologies, 13812) 1:750; anti-GAPDH (Abcam, ab8245) 1:2000; anti-α tubulin (Abcam, ab7291 and Santa Cruz Biotechnology, sc-8035) 1:1000 for both; anti-vinculin (Santa Cruz Biotechnology, sc-73614) 1:2000. Secondary antibodies used: anti-rabbit IgG-HRP (GE Healthcare, NA934) and anti-mouse IgG-HRP (GE Healthcare, NA931V), both at 1:3000 dilutions.

Manohar S., Yu Q., Gygi S.P, & King R.W. (2020). The Insulin Receptor Adaptor IRS2 is an APC/C Substrate That Promotes Cell Cycle Protein Expression and a Robust Spindle Assembly Checkpoint. Molecular & Cellular Proteomics : MCP, 19(9), 1450-1467.

+ Open protocol

+ Expand

Immunoblotting and Immunocytochemistry Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

Anti-NF45 (Bethyl), Anti-N90 (Bethyl), Anti-NUF2 (Abcam), Anti-CDK1 (Santa Cruz), Anti-Cyclin A2 (Santa Cruz), Anti-Survivin (Novus Biologicals), Anti-Tubulin (Sigma-Aldrich), Anti-Actin (Sigma-Aldrich), Anti-Myc (Sigma-Aldrich), Anti-UPF1 (MBL), phosphor-H3 Ser10 (Cell Signaling), Nocodazole (Sigma-Aldrich), Puromycin (Sigma-Aldrich), DAPI vectashield (Vectorlabs), TexasRed-Phalloidin (Invitrogen). All secondary horseradish peroxidase (HRP)-conjugated antibodies used for immunoblotting were purchased from Chemicon.

Nourreddine S., Lavoie G., Paradis J., Ben El Kadhi K., Méant A., Aubert L., Grondin B., Gendron P., Chabot B., Bouvier M., Carreno S, & Roux P.P. (2020). NF45 and NF90 Regulate Mitotic Gene Expression by Competing with Staufen-Mediated mRNA Decay. Cell reports, 31(7).

+ Open protocol

+ Expand

Cell Cycle Regulation Protein Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Anti-FLAG M2 and anti-β-actin antibodies were purchased from Sigma-Aldrich Chemicals. Anti-p21^waf1, anti-cyclin D1, anti-p53, anti-BCL2, and anti-Ki-67 antibodies were obtained from Dako (Glostrup, Denmark). Anti-p27^kip1 and anti-BAX antibodies were from BD Biosciences (San Jose, CA, USA). Anti-MDM2 and anti-S100A1 were from Abcam (Cambridge, MA, USA). Anti-cleaved caspase-3 and anti-cleaved poly (ADP-ribose) polymerase 1 (PARP1)(Asp214)(D64E10) were from Cell Signaling Technology (Danvers, MA, USA). Anti-cyclin B1 and anti-cyclin A2 antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA), and Novocastra (Newcastle, UK), respectively.
Rapamycin, aphidicolin, and nocodazole for synchronization of cells at G1, early S, and G2/M phases, respectively, were obtained from Calbiochem (Cambridge, MA, USA). Adriamycin (ADR) and Nutlin-3A were from Sigma-Aldrich Chemicals.

Nakagawa M., Higuchi S., Hashimura M., Oguri Y., Matsumoto T., Yokoi A., Ishibashi Y., Ito T, & Saegusa M. (2022). Functional interaction between S100A1 and MDM2 may modulate p53 signaling in normal and malignant endometrial cells. BMC Cancer, 22, 184.

+ Open protocol

+ Expand

Cell Synchronization and Protein Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols

Anti-FLAG M2 and anti-β-actin antibodies were purchased from Sigma-Aldrich Chemicals. Anti-p21 waf1 , anti-cyclin D1, anti-p53, anti-BCL2, and anti-Ki-67 antibodies were obtained from Dako (Copenhagen, Denmark). Anti-p27 kip1 and anti-BAX antibodies were from BD Biosciences (San Jose, CA, USA). Anti-MDM2 and anti-S100A1 were from Abcam (Cambridge, MA, USA). Anti-cleaved caspase-3 and anticleaved poly (ADP-ribose) polymerase 1 (PARP1)(Asp214)(D64E10) were from Cell Signaling (Danvers, MA, USA). Anti-cyclin B1 and anti-cyclin A2 antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA), and Novocastra (Newcastle, UK), respectively. Rapamycin, aphidicolin, and nocodazole for synchronization of cells at G1, early S, and G2/M phases, respectively, were obtained from Calbiochem (Cambridge, MA, USA). Adriamycin (ADR) and Nutlin-3A were from Sigma-Aldrich Chemicals.
Reverse transcription (RT)-PCR cDNA was synthesized from 2 µg of total RNA. Ampli cation by RT-PCR was carried out in the exponential phase to allow comparison among cDNA synthesized from identical reactions using speci c primers (Table 1). Primers for the GAPDH gene were also used as described previously [13, 16] .

Nakagawa M., HIGUCHI S., Hashimura M., Oguri Y., Matsumoto T., Yokoi A., Ishibashi Y., Ito T., & Saegusa M. (2021). Functional Interaction Between S100A1 and MDM2 May Modulate p53 Signaling in Normal and Malignant Endometrial Cells.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!