For Immunoblot, whole cell lysate was obtained with low-salt lysis buffer after virus infection for indicated time points. Protein samples were mixed with the 5X loading buffer (Cell Signaling Technology) and resolved by SDS-PAGE. After electrophoresis, protein was transferred to polyvinylidene fluoride membranes (Bio-Rad Laboratories) and then incubated with appropriate antibody. LumiGlo Chemiluminescent Substrate System (KPL) was used to detect specific band of certain protein. Antibodies used in immunoblot were listed as follows. Anti-IRF3 rabbit polyclonal antibody, goat anti–mouse IgG-HRP and goat anti–rabbit IgG-HRP antibodies were purchased from Santa Cruz Biotechnology Inc. Anti-phospho-IRF3 (Ser396) rabbit monoclonal antibody, anti-RIG-I rabbit monoclonal antibody, anti-p38 MAPK rabbit polyclonal antibody, anti-phospho-p38 MAPK (Thr180/Tyr182) rabbit polyclonal antibody, anti-SAPK/JNK rabbit polyclonal antibody, anti-phospho-SAPK/JNK (Thr183/Tyr185) rabbit polyclonal antibody, anti-TBK1/NAK (D1B4) rabbit monoclonal antibody, anti-phospho-TBK1/NAK (Ser172) rabbit monoclonal antibody, IκBα muse monoclonal antibody were purchased from Cell Signaling Technology. Anti-IRF1 mouse polyclonal antibody was bought from abcam®.
Anti phospho sapk jnk thr183 tyr185 rabbit polyclonal antibody
Manufactured by Cell Signaling Technology
Sourced in United States
Anti-phospho-SAPK/JNK (Thr183/Tyr185) rabbit polyclonal antibody is a lab equipment product that detects phosphorylation of SAPK/JNK at Thr183 and Tyr185 residues.
Automatically generated - may contain errors
Lab products found in correlation
Goat anti mouse igg hrp, by Santa Cruz Biotechnology (1 mentions)
Goat anti rabbit igg hrp antibody, by Santa Cruz Biotechnology (1 mentions)
Loading buffer, by Cell Signaling Technology (1 mentions)
Anti irf3 rabbit polyclonal antibody, by Santa Cruz Biotechnology (1 mentions)
Rabbit polyclonal anti p38 mapk antibody, by Cell Signaling Technology (1 mentions)
Polyvinylidene fluoride membrane, by Bio-Rad (1 mentions)
Anisomycin, by Merck Group (1 mentions)
Irdye 680 goat anti mouse igg, by LI COR (1 mentions)
Sb203580, by Merck Group (1 mentions)
Irdye 800cw goat anti rabbit, by LI COR (1 mentions)
Blasticidin s, by InvivoGen (1 mentions)
Chir 99021, by Focus Biomolecules (1 mentions)
5z 7 oxozeaenol, by Merck Group (1 mentions)
Imd 0354, by Merck Group (1 mentions)
Anti phospho p38 mapk thr180 tyr182 antibody, by Cell Signaling Technology (1 mentions)
Jnk inhibitor 8, by Merck Group (1 mentions)
Epidermal growth factor (egf), by Merck Group (1 mentions)
Poly 1 c, by Merck Group (1 mentions)
Puromycin, by InvivoGen (1 mentions)
2 protocols using anti phospho sapk jnk thr183 tyr185 rabbit polyclonal antibody
1
Immunoblot Analysis of Virus-Induced Signaling
2
Immunoblotting and Immunocytochemistry Antibody Protocols
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The following primary and secondary antibodies were used for immunoblotting analysis and immunocytochemistry: anti‐TAK1 rabbit polyclonal antibody, anti‐phospho‐SAPK/JNK (Thr183/Tyr185) rabbit polyclonal antibody, and anti‐phospho‐p38 MAPK (Thr180/Tyr182) antibody (Cell Signaling Technology, Danvers, MA, USA); anti‐TNF Receptor I rabbit polyclonal antibody (Abcam, Cambridge, UK); anti‐IL‐1 mouse mAb (Santa Cruz Biotechnology, Dallas, TX, USA); anti‐α‐tubulin (DM1A) mouse mAb (Merck Millipore, Billerica, MA, USA); IRDye 680 goat anti‐mouse IgG and IRDye 800CW goat anti‐rabbit (LI‐COR Biosciences, Lincoln, NE, USA); and Alexa Fluor anti‐mouse F4/80 antibody (BioLegend, San Diego, CA, USA). Mouse IL‐1β and human TNF‐α were from Wako (Osaka, Japan).
Blasticidin S and puromycin were purchased from InvivoGen (San Diego, CA). (5z)‐7‐oxozeaenol, JNK inhibitor VIII, anisomycin, dibutyryl cyclic AMP, and TPA were purchased from Merck Millipore. Epidermal growth factor, SB203580, IMD‐0354, and PolyI:C were purchased from Sigma‐Aldrich (St. Louis, MO, USA). The GSK‐3β inhibitor, CHIR‐99021, was purchased from Focus Biomolecules (Plymouth Meeting, PA, USA).
Blasticidin S and puromycin were purchased from InvivoGen (San Diego, CA). (5z)‐7‐oxozeaenol, JNK inhibitor VIII, anisomycin, dibutyryl cyclic AMP, and TPA were purchased from Merck Millipore. Epidermal growth factor, SB203580, IMD‐0354, and PolyI:C were purchased from Sigma‐Aldrich (St. Louis, MO, USA). The GSK‐3β inhibitor, CHIR‐99021, was purchased from Focus Biomolecules (Plymouth Meeting, PA, USA).
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