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Super signal west fempto chemiluminescent ecl

Manufactured by Thermo Fisher Scientific
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The Super Signal West Fempto chemiluminescent ECL is a laboratory equipment product developed by Thermo Fisher Scientific. It is a chemiluminescent substrate used for the detection of protein targets in Western blot analysis.

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Lab products found in correlation

Nupage 4 12 bis tris, by Thermo Fisher Scientific (3 mentions) Hrp conjugated secondary antibody, by Agilent Technologies (3 mentions) Tris acetate gel, by Thermo Fisher Scientific (3 mentions) Nupage sample reducing agent, by Thermo Fisher Scientific (3 mentions) Imagequant 800, by GE Healthcare (2 mentions) Veriblot, by Abcam (2 mentions) Amersham imager 600, by GE Healthcare (2 mentions) Nupage loading buffer, by Thermo Fisher Scientific (2 mentions) Amersham imager, by Cytiva (2 mentions)

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Super Signal (2 citations)

3 protocols using super signal west fempto chemiluminescent ecl

1

Whole Cell Protein Lysate Preparation

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Whole cell protein lysates for cell line validations were prepared using RSB100 (10 mM Tris-HCl pH7.5, 100 mM NaCl, 2.5 mM MgCl2, 0.5% v/v NP-40, 0.5% v/v Triton X-100) freshly supplemented with protease inhibitors (Roche). Samples were denatured by the addition of NuPAGE Loading Buffer (Invitogen) and NuPAGE Sample Reducing Agent (Invitrogen) before boiling at 95°C for 10 minutes. SDS PAGE was carried out on either NuPAGE 4-12% Bis-Tris or 3-8% Tris-Acetate gels (Invitrogen). Western blotting analysis was carried out according to standard protocols with the antibodies listed in key resource table and HRP conjugated secondary antibodies (Agilent). Bands were visualized by Super Signal West Fempto chemiluminescent ECL (Thermo) and captured using an ImageQuant 800 imaging system (GE Healthcare). Images were processed using ImageJ (v1.51) (Schneider et al., 2012 (link)).
Gerlach P., Garland W., Lingaraju M., Salerno-Kochan A., Bonneau F., Basquin J., Jensen T.H, & Conti E. (2022). Structure and regulation of the nuclear exosome targeting complex guides RNA substrates to the exosome. Molecular Cell, 82(13), 2505-2518.e7.
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2

Whole Cell Protein Lysis and Western Blotting

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Whole cell protein lysates were prepared using either RSB100 (10 mM Tris-HCl pH 7.5, 100 mM NaCl, 2.5 mM MgCl2, 0.5% NP-40, 0.5% Triton X-100) or TOPEX+ buffer (Riising et al., 2014 (link)) (50 mM Tris-HCl pH 7.5, 300 mM NaCl, 0.5% Triton X-100, 1% SDS, 33.3 U/mL Benzonase) freshly supplemented with protease inhibitors (Roche). Samples were denatured by the addition of NuPAGE Loading Buffer (Invitrogen) and NuPAGE Sample Reducing Agent (Invitrogen) before boiling at 95°C for 10 min. SDS-PAGE was carried out on either NuPAGE 4%–12% Bis-Tris or 3%–8% Tris-Acetate gels (Invitrogen). Western blotting analysis was carried out according to standard protocols with the antibodies listed in the key resources table and HRP conjugated secondary antibodies (Agilent) or Veriblot (Abcam). Bands were visualized by Super Signal West Fempto chemiluminescent ECL (Thermo) and captured using an Amersham Imager 600 or ImageQuant 800 imaging systems (GE Healthcare). Images were processed and quantified using ImageJ (v1.51) (Schneider et al., 2012 (link)).
Garland W., Müller I., Wu M., Schmid M., Imamura K., Rib L., Sandelin A., Helin K, & Jensen T.H. (2022). Chromatin modifier HUSH co-operates with RNA decay factor NEXT to restrict transposable element expression. Molecular cell, 82(9), 1691-1707.e8.
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3

Whole Cell Protein Lysis and Western Blotting

Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole cell protein lysates were prepared using either RSB100 (10 mM Tris-HCl pH 7.5, 100 mM NaCl, 2.5 mM MgCl2, 0.5% NP-40, 0.5% Triton X-100) or TOPEX+ buffer (Riising et al., 2014 (link)) (50 mM Tris-HCl pH 7.5, 300 mM NaCl, 0.5% Triton X-100, 1% SDS, 33.3 U/mL Benzonase) freshly supplemented with protease inhibitors (Roche). Samples were denatured by the addition of NuPAGE Loading Buffer (Invitrogen) and NuPAGE Sample Reducing Agent (Invitrogen) before boiling at 95°C for 10 min. SDS-PAGE was carried out on either NuPAGE 4%–12% Bis-Tris or 3%–8% Tris-Acetate gels (Invitrogen). Western blotting analysis was carried out according to standard protocols with the antibodies listed in the key resources table and HRP conjugated secondary antibodies (Agilent) or Veriblot (Abcam). Bands were visualized by Super Signal West Fempto chemiluminescent ECL (Thermo) and captured using an Amersham Imager 600 or ImageQuant 800 imaging systems (GE Healthcare). Images were processed and quantified using ImageJ (v1.51) (Schneider et al., 2012 (link)).
Garland W., Müller I., Wu M., Schmid M., Imamura K., Rib L., Sandelin A., Helin K, & Jensen T.H. (2022). Chromatin modifier HUSH co-operates with RNA decay factor NEXT to restrict transposable element expression. Molecular cell, 82(9), 1691-1707.e8.
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