Anti nestin mab353

Manufactured by Merck Group

Sourced in Germany

Anti-nestin (MAB353) is a monoclonal antibody that binds to the intermediate filament protein nestin. Nestin is a marker for neural stem and progenitor cells. The antibody can be used for the identification and characterization of nestin-expressing cells.

Automatically generated - may contain errors

Lab products found in correlation

6 protocols using anti nestin mab353

Immunohistochemical Analysis of Tumor Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

All immuno-labeling were performed by the Lausanne Mouse Pathology Facility. Hematoxylin and eosin (H&E) staining was performed on all subcutaneous and orthotopic tumors. Immunohistochemistry (IHC) was performed using mouse monoclonal Ab anti-TH (MAB318, Millipore), rabbit polyclonal Ab anti-ALK (51-3900, Invitrogen), anti-Tyr (a gift from Bhushan Sarode, EPFL), anti-CD99 (Orb13719, Biorbyt), anti-Ncam1 (14255-1-AP, Proteintech), rat monoclonal Ab anti-CD44 (550538, BD Pharmingen), anti-Ptprc (CD45) (550539, BD Pharmingen), anti-nestin (MAB353, Millipore), anti-Ki67 (M7249, DAKO), anti-Phox2b (H-20, Santa Cruz Biotechnology).
Ki67 proliferation index was determined by analyzing 5 fields imaged at 40x magnification per tumor section in 4 different tumors from each group. Sections were imaged using a Zeiss Axio LabA1 microscope and Axiovision Rel. 4.5 imaging software.

Montavon G., Jauquier N., Coulon A., Peuchmaur M., Flahaut M., Bourloud K.B., Yan P., Delattre O., Sommer L., Joseph J.M., Janoueix-Lerosey I., Gross N, & Mühlethaler-Mottet A. (2014). Wild-type ALK and activating ALK-R1275Q and ALK-F1174L mutations upregulate Myc and initiate tumor formation in murine neural crest progenitor cells. Oncotarget, 5(12), 4452-4466.

+ Open protocol

+ Expand

Antibody Profiling for Cartilage Research

Check if the same lab product or an alternative is used in the 5 most similar protocols

Anti–type I collagen cleavage site antibody (#0217-050) was purchased from ImmunoGlobe. Anti-Flag antibody (F3165) and anti-vinculin antibody (V9131) were purchased from Sigma-Aldrich. Normal mouse IgG (sc-2025), normal rabbit IgG (sc-2027), anti–phospho-JNK Thr¹⁸³/Tyr¹⁸⁵ antibody (sc-6254), anti–phospho-p38 Tyr¹⁸² antibody (sc-166182), anti-COL3A1 antibody (sc-271249), anti-actin antibody (sc-1615), anti-SOX9 antibody (sc-166505), and anti-SSEA4 (sc-21704) were purchased from Santa Cruz Biotechnology. Anti-fibronectin antibody (A0245) was purchased from DAKO. Anti-CTRP3 antibody (ab36870), anti-MMP13 (ab51072), anti-CD146 (ab75769), and anti–chondroitin sulfate (ab11570) were purchased from Abcam. Anti–phospho-Akt Ser⁴⁷³ (#9271), anti-Akt (pan) (#4685), and anti–phospho-Erk1/2 Thr²⁰²/Tyr²⁰⁴ (#4377) were purchased from Cell Signaling Technology. Anti-ACAN antibody (AB1031), anti–type II collagen antibody (MAB8887), and anti-nestin (MAB353) were purchased from Millipore. Rabbit anti-mouse IgG + IgM (H&L) conjugated with DyLight 488 (711-065-151) and goat anti-rabbit IgG + IgM (H&L) conjugated with DyLight 594 (111-585-003) were purchased from Jackson ImmunoResearch. Full-size immunoblot images are provided in fig. S19.

Cho Y., Kim H.S., Kang D., Kim H., Lee N., Yun J., Kim Y.J., Lee K.M., Kim J.H., Kim H.R., Hwang Y.I., Jo C.H, & Kim J.H. (2021). CTRP3 exacerbates tendinopathy by dysregulating tendon stem cell differentiation and altering extracellular matrix composition. Science Advances, 7(47), eabg6069.

+ Open protocol

+ Expand

Characterization of Pluripotent Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Di-hydrotashinone (DT, 1 µM, D0947, Sigma-Aldrich); actinomycin-D (1 μg/ml, CID 2019, Sigma-Aldrich); thiazolyl blue tetrazolium bromide – MTT (M2128, Sigma-Aldrich); anti-Nestin (MAB353, Millipore); anti-DCX (sc8066, Santa Cruz); anti-HuR (390,600, Invitrogen); anti-βTubIII (MMS-435P, Covance); anti-MAP2 (Ab5622, Millipore); anti-GFAP (PRB571c, Covance); anti-GalC (Mab 342, Millipore); anti-Olig2 (Ab9610, Millipore); pluripotent stem cells 4-Marker Immunocytochemistry Kit (A24881, Thermo Fisher); 4,6, diamino-2-phenyl-l-indole di-hydrocloride–DAPI (D259542, Sigma-Aldrich), Alexa Fluor® 488 (Life Technologies) Alexa Fluor® 546 (Life Technologies), cresyl-violet (Sigma-Aldrich), Peroxidase AffiniPure Goat Anti-Rabbit/Mouse IgG (Jackson).

Carelli S., Giallongo T., Rey F., Latorre E., Bordoni M., Mazzucchelli S., Gorio M.C., Pansarasa O., Provenzani A., Cereda C, & Di Giulio A.M. (2019). HuR interacts with lincBRN1a and lincBRN1b during neuronal stem cells differentiation. RNA Biology, 16(10), 1471-1485.

+ Open protocol

+ Expand

Molecular Mechanisms of Alzheimer's Disease

Check if the same lab product or an alternative is used in the 5 most similar protocols

Levodopa, bromocriptine, carbidopa, L-685,458, TAPI-1, Y-27632, and Forskolin (FSK) were purchased from Selleck Chemicals (Houston, TX, USA). Piribedil was from Tocris Bioscience (Bristol, UK). β-Secretase Inhibitor IV (BSI IV) was from Calbiochem (Hayward, CA, USA). Recombinant human BDNF, GNDF, IGF-I were from Peprotech (Rocky Hill, NJ, USA). cAMP and L-Ascorbic acid were from Sigma (St Louis, MO, USA). CellTiter-Glo was from Promega (Madison, WI, USA) and Effectene Transfection Reagent was purchased from QIAGEN (Hilden, Germany). Immunoblotting was performed with the following antibodies: anti-PS1 N-terminus (1–65) (PRB-354P, Covance, Davis, CA, USA); anti-APH1aL C-terminus (245–265) (PRB-550P, Covance); anti-NCT (N1660, Sigma); anti-Pen2 (P5622, Sigma); anti-BACE1 N-termimus (AP7774b, Abgent, Suzhou, China); anti-APP-CTF (A8717, Sigma); anti-sAPPα (secreted Amyloid Precursor Protein-α) (11088, IBL, Hokkaido, Japan); anti-HA (H6908, Sigma); anti-actin (A2066, Sigma); anti-D₂R (sc-9913, Santa Cruz Biotechnology, Santa Cruz, CA, USA). Immunostaining was performed with the following antibodies: anti-nestin (MAB353, Milipore, Darmstadt, Germany); anti-Sox2 (sc-17320, Santa Cruz); anti-Ki67 (556003, BD Transduction Laboratories, San Jose, CA, USA), and anti-Doublecortin (DCX) (sc-8066, Santa Cruz).

Lu J., Li X., Wang Q, & Pei G. (2017). Dopamine D2 receptor and β-arrestin 2 mediate Amyloid-β elevation induced by anti-parkinson’s disease drugs, levodopa and piribedil, in neuronal cells. PLoS ONE, 12(3), e0173240.

+ Open protocol

+ Expand

Immunostaining Protocols for Neural Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

XAV939 was purchased from Tocris Bioscience. BrdU and fluoxetine were from Sigma-Aldrich. The following antibodies were used for immunostaining: anti-Axin (A0481, Sigma-Aldrich), anti-Nestin (MAB353, Millipore), anti-Prox1 (sc-8025, Santa Cruz Biotechnology), anti-NeuN (MAB377B, Millipore), anti-doublecortin (DCX, AB2253; Millipore), anti-BrdU (OBT0030G, AbD Serotec), and anti-GFP (clone 3E6) Alexa Fluor 488-, 546-, and 647-conjugated secondary antibodies (Thermo Fisher).

Chen W.W., Fu W.Y., Su Y.T., Fang W.Q., Fu A.K, & Ip N.Y. (2019). Increased Axin expression enhances adult hippocampal neurogenesis and exerts an antidepressant effect. Scientific Reports, 9, 1190.

+ Open protocol

+ Expand

Immunohistochemistry of Bone Tissue Sections

Check if the same lab product or an alternative is used in the 5 most similar protocols

Frozen tissue sections prepared from 4% paraformaldehyde-fixed and decalcified bones were thawed at room temperature and then rehydrated with PBS. The slides were stained with the following antibodies (eBiosciences, San Diego, unless otherwise noted) following standard procedures: anti-Osteopontin (Abcam), anti-Nestin (MAB353, Millipore), anti-Gr-1 (RB6-8C5), anti-Mac-1 (M1/70), anti-B220 (RA3-6B2), anti-Ter-119 (TER-119), anti-CD3 (145-2C11, BD Biosciences), anti-CD115 (AFS98), anti-CD150 (TC15-12F12.2, BD Biosciences), anti-CD31 (MEC13.3, Biolegend), anti-CD48 (HM48-1), and anti-CD41 (eBioMWReg30) antibodies. Images were acquired using Olympus Confocal Laser Scanning Biological Microscope FV1000 equipped with four lasers ranging from 405 to 635 nm. Images were processed with ImageJ software.

Dong L., Yu W.M., Zheng H., Loh M.L., Bunting S.T., Pauly M., Huang G., Zhou M., Broxmeyer H.E., Scadden D.T, & Qu C.K. (2016). Leukaemogenic effects of Ptpn11 activating mutations in the stem cell microenvironment. Nature, 539(7628), 304-308.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!