Graph pad 5.0 for windows

All statistical analyses were performed using Graph Pad® 5.0 for Windows. Data were tested for normality by the selection of parametric and non-parametric tests. Data were analyzed by a Two-way analysis of variance for repeated measures (Two-way RM ANOVA) followed by a Bonferroni’s multiple comparison test or Student’s t-test, as required. Differences were considered significant only when P-values were less than 0.05.

The data was collected and graphed using Microsoft Excel^®. Data was then exported to Graph Pad 5.0 for Windows (GraphPad Software, La Jolla, CA, USA) for statistical analysis. The effects of plant extracts on C. albicans inoculated onto solid agar media, liquid broth and during biofilm formation was analyzed using one-way analysis of variance (ANOVA) using Dunnett’s Multiple Comparison Test. P value <0.05 was considered as significant.

To check the normal distribution of the samples, we used the Kolmogorov-Smirnov and the Shapiro-Wilk test. For variables with normal distribution, we used the unpaired t-test for comparison; in the absence of a normal distribution, we used the Mann-Whitney test. The quantitative variables were represented as mean and standard deviation. For qualitative variables, we used the Fisher and Chi-square tests. The level of significance adopted was 5% with a power of 80%. In addition, we ran a multivariate analysis to assess the prediction of the covariables: age, time and cause of infertility and the number of visits to the clinic on the number of mature oocytes. We ran the statistical analysis with the aid of the SPSS 16.0 for Windows (SPSS Inc., Chicago, Illinois, USA), SAS 9.0 (SAS Institue Inc., North Caroline University, USA) and the GraphPad 5.0 for Windows (GraphPad Software, San Diego, California, USA).