7500ht instrument

Manufactured by Thermo Fisher Scientific

Sourced in United States

The 7500HT instrument is a laboratory equipment designed for real-time PCR (Polymerase Chain Reaction) analysis. It is capable of performing quantitative, qualitative, and allelic discrimination assays. The 7500HT instrument provides precise temperature control and detection of fluorescent signals during the PCR process, enabling researchers to analyze genetic sequences and expression levels with accuracy.

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Lab products found in correlation

Rneasy mini kit, by Qiagen (1 mentions) 2100 bioanalyzer, by Agilent Technologies (1 mentions) Nanodrop spectrophotometer, by Thermo Fisher Scientific (1 mentions) Taqman assay, by Thermo Fisher Scientific (1 mentions) Taqman gene expression assay, by Thermo Fisher Scientific (1 mentions)

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ABI 7500HT instrument (6 citations)

2 protocols using 7500ht instrument

Comprehensive Gene Expression Analysis

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Total RNA was extracted from cell cultures by RNeasy Mini Kit (Qiagen). The RNA quality was assessed using 2100 Bioanalyzer (Agilent) and NanoDrop spectrophotometer (Thermo Scientific). qRT-PCR analyses was performed using 7500HT instrument (Applied Biosystems). Total RNA was reverse transcribed with specific primers and then qRT-PCR was performed with the following TaqMan Assays (Thermo Fisher Scientific): Mm02619580_g1 for Actb, Hs04260367_gH for OCT4, Hs04234836_s1 for SOX2, Hs01002915_g1 for CD40, Hs00977641_m1 for CD166, Hs06633377_s1 for CD90, Hs00159686_m1 for CD73, Hs04187831_g1 for NES, Hs00161904_m1 for SNAI2, Hs02718934_s1 for BDNF, Hs00900055_m1 for VEGF, Hs00171458_m1 for NGF, Hs01931883_s1 for GDNF and Hs04940643_m1 for miR-514a-3p. All target genes were assayed in triplicates on each plate and all qPCR experiments were independently repeated five times.

Stančin P., Song M.S., Alajbeg I, & Mitrečić D. (2022). Human Oral Mucosa Stem Cells Increase Survival of Neurons Affected by In Vitro Anoxia and Improve Recovery of Mice Affected by Stroke Through Time-limited Secretion of miR-514A-3p. Cellular and Molecular Neurobiology, 43(5), 1975-1988.

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qPCR Analysis of Cell Cycle Regulators

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Taqman Gene Expression assays were purchased from ThermoFisher Scientific (Waltham, MA, USA): ATR (Hs00992123_m1), ATRIP (Hs04335019_s1), CDC6 (HS00154374_m1), CDT1 (Hs00925491_g1), CHEK1/CHK1 (Hs00967506_m1), CHEK2/CHK2 (Hs00200485_m1), CLSPN/Claspin (Hs00898637_m1), ETAA1(Hs00936568_m1), GMNN/Geminin (Hs00992846_m1), MRE11A (Hs00967437_m1), NBN/NBS1(Hs01039845_m1), RAD17 (Hs00607830_m1), RPA2 (Hs00989573_g1), TOPBP1 (Hs00199775_m1), GAPDH (Hs02786624_g1). qPCR reactions were carried out on a 7500 HT instrument (Applied biosystems, Foster City, CA, USA). The amplified transcript level of each specific gene was normalized to that of GAPDH. Results shown represent average of three independent experiments.

Zhang J., Dulak A.M., Hattersley M.M., Willis B.S., Nikkilä J., Wang A., Lau A., Reimer C., Zinda M., Fawell S.E., Mills G.B, & Chen H. (2018). BRD4 facilitates replication stress-induced DNA damage response. Oncogene, 37(28), 3763-3777.

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