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2 cl ib meca

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2-Cl-IB-MECA is a selective A3 adenosine receptor agonist. It is a laboratory reagent used in research applications.

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Lab products found in correlation

Cgs21680, by Bio-Techne (1 mentions) Ib meca, by Bio-Techne (1 mentions) Bicuculline methochloride, by Bio-Techne (1 mentions) Cyclothiazide, by Bio-Techne (1 mentions) Tetrodotoxin citrate, by Bio-Techne (1 mentions) Dpcpx, by Bio-Techne (1 mentions) Mrs1523, by Merck Group (1 mentions) Sch58261, by Bio-Techne (1 mentions) Psb 10 hydrochloride, by Bio-Techne (1 mentions) Lipopolysaccharides lps from escherichia coli o111 b4, by Merck Group (1 mentions) Interferon γ ifn γ, by Merck Group (1 mentions)

Close spelling variants of this product

IB-MECA (6 citations) Cl-IB-MECA (4 citations)

4 protocols using 2 cl ib meca

1

Characterization of A3 Adenosine Receptor

Cited 1 time
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HEK293T cells (passage 20) were kindly provided by Prof. O. De Wever
(Laboratory of Experimental Cancer Research, Department of Radiation Oncology
and Experimental Cancer Research, Ghent University Hospital, Belgium). The human
A3AR construct (NM_000677.2,
transcript variant 2 of the ADORA3 gene) and human βarr2
construct (NM_004313) were purchased from
Origene Technologies (Rockville, MD, USA). DOTAP Liposomal Transfection Reagent
was purchased from Sigma Aldrich (Steinheim, Germany), and the anti-dNGFR
antibody was purchased from Chromaprobe (Maryland Heights, MO, USA). Reference
agonists 2-Cl-IB-MECA, IB-MECA, CGS21680 and NECA were purchased from Tocris
Bioscience (Bio-techne, Abingdon, UK). All other chemicals and reagents used
were purchased from the same suppliers as described previously [18 (link)] or synthesized at NIDDK, National Institutes of
Health as reported [19 (link)–33 (link)].
Storme J., Tosh D.K., Gao Z.G., Jacobson K.A, & Stove C.P. (2018). Probing Structure-Activity Relationship in β-Arrestin2 Recruitment of Diversely Substituted Adenosine Derivatives. Biochemical pharmacology, 158, 103-113.
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2

Pharmacological Reagents for Neurological Studies

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SCH5821 (stock solution 10 mM in DMSO), DPCPX (stock solution 5 mM in DMSO), 2-Cl-IBMECA (stock solution 10 mM in DMSO), Bicuculline methochloride (stock solution 100 mM), AMPA (stock solution 100 mM), cyclothiazide (stock solution 50 mM in DMSO) and Tetrodotoxin citrate (stock solution 1 mM) were purchased from Tocris Bioscience, Bristol, UK. MRS1523 (stock solution 100 mM in DMSO) and all other drugs used were purchased from Sigma–Aldrich, Milan, Italy. Where not indicated, all drugs were dissolved in water.
Di Angelantonio S., Bertollini C., Piccinin S., Rosito M., Trettel F., Pagani F., Limatola C, & Ragozzino D. (2015). Basal adenosine modulates the functional properties of AMPA receptors in mouse hippocampal neurons through the activation of A1R A2AR and A3R. Frontiers in Cellular Neuroscience, 9, 409.
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3

Modulating Microglia Response to Hyperbaric Pressure

Cited 2 times
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The cells were challenged with EHP (70 mmHg above atmospheric pressure) for 4 or 24 h, as previously detailed [10 (link),26 (link),28 (link)]. Microglia cultures were incubated with 1 µM 2-chloro-N6-(3-iodobenzyl)-adenosine-5′-N-methyluronamide (2-Cl-IB-MECA, Tocris Bioscience, Cambridge, UK), an A3R selective agonist 45 min before exposing the cells to EHP.
Ferreira-Silva J., Aires I.D., Boia R., Ambrósio A.F, & Santiago A.R. (2020). Activation of Adenosine A3 Receptor Inhibits Microglia Reactivity Elicited by Elevated Pressure. International Journal of Molecular Sciences, 21(19), 7218.
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4

Pharmacological reagents for cell signaling

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PSB 777 ammonium salt, 2-Cl-IB-MECA, SCH 58261, and PSB 10 hydrochloride were purchased from Tocris Bioscience (Bristol, UK). Concentrated (10 mM) stock solutions prepared in DMSO were stored at −20 °C. In each experimental session, aliquots of concentrated solutions of compounds were thawed and conveniently diluted in the appropriate experimental solution. Lipopolysaccharides from Escherichia coli O111:B4 (LPS) and Interferon-γ (IFN-γ) were purchased from SigmaAldrich (St. Louis, MO, USA).
Lillo A., Raïch I., Lillo J., Pérez-Olives C., Navarro G, & Franco R. (2022). Expression of the Adenosine A2A-A3 Receptor Heteromer in Different Brain Regions and Marked Upregulation in the Microglia of the Transgenic APPSw,Ind Alzheimer’s Disease Model. Biomedicines, 10(2), 214.
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