Hemicellulase

The shelled hemp seeds used in this experiment were purchased from Bama, Guangxi, China. They were thoroughly hulled, cleaned, and dried at room temperature until a uniform weight was reached. Prior to extraction, they were ground in a mill, sieved through a 60-mesh sieve, and stored at 4 °C until further use.
Cellulase (EC 3.2.1.1, activity 400,000 U/g, optimum pH range 3.5–5.5, temperature range 30–55 °C), hemiCellulase (EC 3.1.1.73, activity 20,000 U/g, optimum pH range 4.0–8.0, temperature range 30–50 °C, from Bacillus subtilis), pectinase (EC 3.2.1.15, activity 500,000 U/g, optimal pH range 3.5–5.5, temperature range 30–55 °C) were purchased from Shanghai Yuan Ye Biotechnology Co, Ltd, Shanghai, China. Neutral protease (EC 3.4.24.28, activity 50,000 U/g, optimal pH range 6.0–8.0, temperature range 30–50 °C, from Bacillus subtilis), acid protease (EC 3.4.23.18, activity 50,000 U/g, optimal pH range 2.0–4.0, temperature range 35–55 °C, from Aspergillus niger), and alkaline protease (EC 3.4.21.14, activity 200,000 U/g, optimal pH range 9.0–11.0, temperature range 40–60 °C, from Bacillus licheniformis). A mixture of Δ⁹-THC, CBD and CBN, and a mixture of fatty acid methyl esters (FAME), were purchased from Sigma Aldrich Co., Ltd Saint Louis, MO, USA. The other chemicals were used as analytical reagents and were purchased from Aladdin Reagent Co., Ltd. (Shanghai, China).