Axiovision camera and software

Manufactured by Zeiss

Sourced in United States

AxioVision is a camera and software package from ZEISS that is designed for microscopy applications. It provides image acquisition, processing, and analysis capabilities for a wide range of microscopy techniques. The core function of AxioVision is to capture high-quality digital images from microscope samples and enable further analysis and documentation.

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Lab products found in correlation

Wide field epifluorescence microscope, by Zeiss (1 mentions) Zen 2 lite imaging software profile tool, by Zeiss (1 mentions) Axioskop inverted microscope, by Zeiss (1 mentions) Vectashield with 4 6 diamidino 2 phenylindole dapi, by Vector Laboratories (1 mentions) Fluorescent microscope, by Zeiss (1 mentions)

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AxioVision and Software Axiovision camera AxioVision software AxioVision

3 protocols using axiovision camera and software

Quantitative Fluorescence Imaging of Neurons

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Fluorescent images were obtained on a Carl Zeiss widefield epi-fluorescence microscope via AxioVision camera and software. Low-magnification images for stage analyses were taken at 20× while high-magnification images for neuron measurements were taken at 40× or 100×. Image analysis was completed through ImageJ software. We used the NeuronJ software plugin to trace and measure axons and minor neurites. Axon branches were only counted if they were a minimum of 5 μm. Neurite lengths were measured from the base of the process at the soma to the tip of the Tuj1 stain. Stage 3 neurons’ minor neurites were measured in thickness at 0 μm, 5 μm, and 10 μm from the edge of the soma. Stage 3 neurons’ axons were measured in thickness at 0 μm, 5 μm, 10 μm, and 25 μm from the edge of the soma. For Fig. 7D, images of tubulin in axons were captured using a Deltavision widefield microscope and then deconvolved. The intensity of tubulin staining was measured across the width of the axon at 5 μm, 10 μm, and 25 μm using Zeiss Zen 2 lite imaging software profile tool.

McNeely K.C., Cupp T.D., Little J.N., Janisch K.M., Shrestha A, & Dwyer N.D. (2017). Mutation of Kinesin-6 Kif20b causes defects in cortical neuron polarization and morphogenesis. Neural Development, 12, 5.

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Immunohistochemical Analysis of Tight Junction Proteins

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Sections of paraffin-embedded colon tissues were dewaxed in xylene, rehydrated, and subjected to antigen retrieval in 10 mM citrate buffer. Sections were blocked for 30 min in 1% bovine serum albumin, 0.02% Triton X-100, and 5% normal goat serum (NGS). Serial sections were incubated with rabbit polyclonal ZO-1 antibody (1:200 dilution) and occludin antibody (1:200 dilution), followed by Cy5-conjugated goat anti-rabbit secondary antibody (Jackson ImmunoResearch Laboratories, Inc., West Grove, PA, USA). The sections were mounted using Vectashield with 4′,6-diamidino-2-phenylindole (DAPI) (Vector Laboratories, Burlingame, CA, USA). Images from serial sections were acquired using an Axioskop inverted microscope with an AxioVision camera and software (Zeiss, Thornwood, NY, USA).

Park K.I., Kim K.Y., Oh T.W., Kang D.S., Kim E.K., Yang Y.R., Seo Y.K., Ma J.Y, & Suh P.G. (2017). Phospholipase Cγ1 links inflammation and tumorigenesis in colitis-associated cancer. Oncotarget, 9(5), 5752-5763.

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Quantitative Analysis of Cerebellar Myelination

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Cryo-protected cerebellums were sectioned para-sagittally at 30μm and stored at -20°C in a glycerol/ethylene glycol storage solution. Prior to staining, sections stored at -20°C were left for 30’ at room temperature and were then washed three times in PBS. Sections were incubated in wells overnight at 4°C with mouse anti-calbindin antibody (Swant cat no. 300) made up in PBS with 0.5% Triton X100 and 2% normal goat serum. Secondary antibodies (Dylite 594 anti-mouse) were made up in PBS with 0.5% Triton X100 and 2% normal goat serum and were incubated for 2h at room temperature. Cholera toxin B subunit directly conjugated to alexafluor488 (Invitrogen, Cat ♯ C-34775) used at 1:500 in PBS with 0.5% Triton X100 and 2% BSA. Sections incubated overnight at 4°C. All washing steps were performed three times in PBS. Sections were mounted onto Superfrost slides and allowed to air dry overnight protected from light and dust before mounting in Mowiol. Images of lobules IV and VI (early and late degenerating lobules respectively) were obtained using a Zeiss fluorescent microscope and Zeiss AxioVision camera and software. Measurements of myelin area and length were performed with ImageJ software (Version 1.46r; NIH, USA).

Kirkegaard T., Gray J., Priestman D.A., Wallom K.L., Atkins J., Olsen O.D., Klein A., Drndarski S., Petersen N.H., Ingemann L., Smith D.A., Morris L., Bornæs C., Jørgensen S.H., Williams I., Hinsby A., Arenz C., Begley D., Jäättelä M, & Platt F.M. (2016). Heat Shock Protein-based therapy as a potential candidate for treating sphingolipidoses. Science translational medicine, 8(355), 355ra118.

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