J 810 150s spectropolarimeter

Manufactured by Jasco

Sourced in Japan

The J-810-150S spectropolarimeter is a lab equipment designed for measuring the circular dichroism (CD) and optical rotation of samples. It is capable of analyzing the interaction between light and chiral molecules across a wide range of wavelengths.

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Lab products found in correlation

Rf 5301 pc spectrofluorimeter, by Shimadzu (1 mentions)

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J-810 spectropolarimeter (841 citations) J-810 (385 citations) Jasco-810 spectropolarimeter (131 citations)

3 protocols using j 810 150s spectropolarimeter

CD Spectroscopy of Peptide Samples

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CD spectroscopy was run on a Jasco J-810-150S spectropolarimeter using the following parameters (pH was chosen equal to those of the NMR experiments):
Data from three consecutive scans were averaged and processed to improve the signal to noise ratio. The mean residue ellipticity (MRE in deg cm² dmol^-1) was calculated using the equation

θ_{M R E} = \frac{θ \cdot M_{r}}{c \cdot l \cdot n}

where θ is the measured ellipticity, M_r the molecular weight of the peptide, c the concentration of the sample, l the pathlength of the cuvette and n the number of peptide bonds.

Schrimpf A., Brödje D., Pfefferle P, & Geyer A. (2018). A set of conformationally well-defined L/D-peptide epitopes provides a serological bar code for autoantibody subtypes. PLoS ONE, 13(8), e0201735.

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Circular Dichroism Analysis of Protein Melting

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A Jasco J-810–150S Spectropolarimeter was used to obtain CD spectra and to determine the melting temperatures of the native LtEc, LP, and LPE samples. CD spectra were obtained by diluting native LtEc, LP, and LPE to a concentration of 0.6 μM in 20 mM phosphate buffer. Spectra were aquired at a scan rate of 50 nm/min at 25°C over a range of 190–250 nm. Four consecutive scans were aquired and averaged together to produce the individual spectra shown in Figure 4.
Melting temperatures for each sample were obtained by recording the ellipticity at 222 nm while increasing the temperature from 40°C to 95°C at a rate of 1°C/min. Samples were first diluted in 20 mM phosphate buffer until the molar elipticity at 222 nm was between −100 to −150 mdeg (~110 mM heme). Measurements were taken in 0.5°C increments with a delay time of 10 seconds and a bandwith of 1 nm.

Spivack K., Tucker M., Zimmerman D., Nicholas M., Abdulmalik O., Comolli N, & Elmer J. (2018). Increasing the Stability of Lumbricus terrestris Erythrocruorin via Poly(acrylic acid) Conjugation. Artificial cells, nanomedicine, and biotechnology, 46(SUP2), 1137-1144.

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PCR and Circular Dichroism Analysis

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The PCR amplification was performed on a ETC 811 PCR Instrument (Eastwin Life Sciences, Inc., China). The fluorescence signal was measured on a RF-5301PC spectrofluorimeter (Shimadzu, Japan). The circular dichroism (CD) spectroscopy was obtained from a J-810-150S spectropolarimeter (JASCO International Co. Ltd., Japan).

Li Y., Miao X, & Ling L. (2015). Triplex DNA: A new platform for polymerase chain reaction – based biosensor. Scientific Reports, 5, 13010.

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