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Phusion direct pcr kit

Manufactured by Thermo Fisher Scientific
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Sourced in United Kingdom

The Phusion Direct PCR kit is a reagent designed for performing direct polymerase chain reaction (PCR) amplification from various biological samples without the need for prior DNA extraction. The kit contains a specially formulated DNA polymerase enzyme and buffer system that enables direct PCR amplification from samples such as blood, saliva, and tissue.

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Lab products found in correlation

Phosphate buffered saline (pbs), by Merck Group (4 mentions) Potassium ferrocyanide, by Merck Group (3 mentions) 4 aminobenzoic acid, by Merck Group (3 mentions) Hotstartaq plus, by Qiagen (3 mentions) Dg8tm cartridge, by Bio-Rad (3 mentions) Supermix for probes, by Bio-Rad (3 mentions) Potassium ferricyanide, by Merck Group (3 mentions) Hydrochloric acid (hcl), by Merck Group (3 mentions) Potassium chloride (kcl), by Merck Group (3 mentions) Sodium chloride (nacl), by Merck Group (3 mentions) Sodium nitrate, by Merck Group (3 mentions) Ethanolamine, by Merck Group (3 mentions) N hydroxysuccinimide, by Merck Group (3 mentions) Droplet generation oil, by Bio-Rad (3 mentions) 1 ethyl 3 3 dimethylaminopropyl carbodiimide hydrochloride, by Merck Group (2 mentions) 2 n morpholino ethanesulfonic acid mes, by Merck Group (2 mentions) Deionized water, by Merck Group (2 mentions) Dntp mix, by Qiagen (1 mentions) Hexammineruthenium 3 chloride, by Merck Group (1 mentions) Sodium hydroxide (naoh), by Merck Group (1 mentions)

3 protocols using phusion direct pcr kit

1

Droplet Digital PCR Assay Protocol

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Supermix for probes, Droplet digital PCR (ddPCR) assays, DG8TM cartridges and Droplet Generation Oil were obtained from Bio-Rad Laboratories Ltd., Hertfordshire, UK. Deionized water, sodium chloride, sodium hydroxide, phosphate-buffered saline (PBS), sodium nitrate, 4-aminobenzoic acid, hydrochloric acid, ethanolamine, 2-(N-morpholino) ethanesulfonic acid (MES), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS), hexammineruthenium (III) chloride, potassium ferricyanide, potassium chloride and potassium ferrocyanide were all purchased from Sigma–Aldrich, (Dorset, UK). Two hundred and fifty units of HotStarTaq Plus and dNTP Mix, PCR Grade (200 μL), were purchased from Qiagen, (Manchester, UK). Phusion Direct PCR kit was purchased from thermo fisher scientific (Renfrew, UK).
Attoye B., Baker M.J., Thomson F., Pou C, & Corrigan D.K. (2021). Optimisation of an Electrochemical DNA Sensor for Measuring KRAS G12D and G13D Point Mutations in Different Tumour Types. Biosensors, 11(2), 42.
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2

Droplet digital PCR protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Droplet digital PCR (ddPCR) assays, Supermix for probes, DG8TM cartridges and Droplet Generation Oil were obtained from BioRad Laboratories Ltd., UK. Deionised water, sodium chloride, phosphate buffered saline (PBS), sodium nitrate, 4-aminobenzoic acid, hydrochloric acid, ethanolamine, 2-(N-morpholino) ethanesulfonic acid (MES), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS), potassium ferricyanide, potassium chloride and potassium ferrocyanide were all purchased from Sigma-Aldrich (Dorset, UK). 250 units HotStarTaq Plus and dNTP Mix, PCR Grade (200 μL) were purchased from Qiagen, (Manchester, UK). Phusion direct PCR kit was purchased from thermo scientific, (Renfrew, UK).
Attoye B., Pou C., Blair E., Rinaldi C., Thomson F., Baker M.J, & Corrigan D.K. (2020). Developing a Low-Cost, Simple-to-Use Electrochemical Sensor for the Detection of Circulating Tumour DNA in Human Fluids. Biosensors, 10(11), 156.
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3

Digital PCR Assays for Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
Droplet digital PCR (ddPCR) assays, Supermix for probes, DG8TM cartridges and Droplet Generation Oil were obtained from Bio-Rad Laboratories Ltd., Hertfordshire, UK. Deionized water, sodium chloride, phosphate-buffered saline (PBS), sodium nitrate, 4-aminobenzoic acid, hydrochloric acid, ethanolamine, 2-(N-morpholino) ethanesulfonic acid (MES), 1-ethyl-3-(3dimethylaminopropyl) carbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS), potassium ferricyanide, potassium chloride and potassium ferrocyanide were all purchased from Sigma-Aldrich, (Dorset, UK). Two hundred and fifty units of HotStarTaq Plus and dNTP Mix, PCR Grade (200 μL), were purchased from Qiagen, (Manchester, UK). Phusion Direct PCR kit was purchased from thermo fisher scientific, (Renfrew, UK).
Attoye B., Baker M.J., Pou C., Thomson F., & Corrigan D.K. (2020). Electrochemical DNA Detection Methods to Measure Circulating Tumour DNA for Enhanced Diagnosis and Monitoring of Cancer.
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