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Rabbit anti cavin1

Manufactured by Abcam
Sourced in United Kingdom

Rabbit anti-cavin1 is a primary antibody that recognizes the cavin1 protein. Cavin1, also known as PTRF, is a structural component of caveolae, invaginations of the plasma membrane. This antibody can be used to detect and quantify cavin1 expression in various cell and tissue samples.

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2 protocols using rabbit anti cavin1

1

Antibody Panel for Protein Analysis

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The following antibodies were used: rabbit anti-caveolin1 (BD Biosciences Cat# 610060), mouse anti-βeta-catenin (BD Biosciences Cat# 610153), rabbit anti-cavin1 (Abcam Cat# ab48824), rabbit anti CD2AP (A599 Cell Signalling, Cat# 5478) for WB and mouse anti-CD2AP (Santa Cruz Biotechnology Cat# sc-25272) for immunostaining, YL1-2 anti-alpha tubulin (in-house cell culture supernatant), mouse anti-GFP (Roche Cat# 11814460001), mouse anti-flotillin-2 (BD, 610384), goat anti-EHD2 (Abcam Cat# ab23935), rabbit anti-CSDE1 (N2C1 GeneTex, Cat# GTX116218), mouse anti-TXNRD1 (Novus Biologicals Cat# NBP2-59489), mouse anti-vigilin (LSBio Cat# LS-C342610-100), rabbit anti-PRRC2C (Abcam Cat#ab117790), rat anti-Nav1 (Abcam Cat#ab201920) and mouse anti-myc clone 9E10 (in-house cell culture supernatant). Horseradish peroxidase (HRP)-conjugated secondary antibodies were from DAKO and Streptavidin HRP from Cell Signalling (Cat# 3999). Streptavidin Alexa 647 and Alexa Fluor fluorescent secondary antibodies were from Life Technologies (Thermo Fisher).
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2

Cavin-1, Caveolins, and ERK Signaling

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The following primary antibodies were used: rabbit anti-Cavin-1 (AbCam, Cambridge, UK, 1:3000 dilution) for immunoblotting and immunofluorescence analyses; rabbit anti-Cavin-1 (ThermoScientific, Waltham, MA, USA) for immunoprecipitation assay; rabbit anti-Cav-1 (Santa Cruz Biotechnology, Dallas, TX, USA, 1:1000 dilution); mouse anti-Cav-1 (Zymed-Life Technologies, Monza, Italy, 1:100 dilution); mouse anti-MHC (Santa Cruz Biotechnology, 1:1000 dilution); mouse anti-Cav-2 (BD, Buccinasco, Italy, 1:1000 dilution); mouse anti-Cav-3 (BD, 1:1000 dilution); mouse anti-total and -phosphorylated ERK1/2 (Tyr204) (Santa Cruz Biotechnology, 1:1000 dilution); mouse antialpha-tubulin (Sigma-Aldrich, 1:10 000 dilution).
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