Anti mycn

Primary antibodies anti‐MYCN and anti‐SESN1 were purchased from Abcam (UK), and anti‐MyD88, anti‐GAPDH, and anti‐β‐Tubulin were purchased from Proteintech (Wuhan, Hubei, China). Cell Counting Kit‐8 (CCK‐8) was bought from Bimake (Shanghai, China). Matrigel was bought from Corning (NY, USA). HiSpeed Plasmid Maxi Kit was bought from Qiagen (Germany). jetPRIME was purchased from PolyPlus Transfection Co. (Illkirsch, France). TLR signaling pathway inhibitor Hydroxychloroquine‐d4 sulfate (HCQ) was purchased from MCE (USA).

Radio immunoprecipitation assay (RIPA) lysis buffer (Beyotime, Beijing, China) was utilized to break corresponding samples. Bicinchoninic acid (BCA) protein assay kit (Beyotime) was applied to mensurate the protein concentration. 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and 0.45 μm polyvinylidene fluoride (PVDF) membrane (Millipore, Billerica, MA) were used to separate and transferc target protein, respectively. Following blocking, diluent primary and secondary antibodies (Cell Signaling Technology, Danvers, MA) were utilized to incubate the membranes sequentially. BeyoECL kit (Beyotime) was employed to visualize the protein bands. GAPDH was applied as an endogenous control. The following primary antibodies were utilized: anti-E-cadherin (Cell Signaling Technology), anti-vimentin (Cell Signaling Technology), anti-snail (Cell Signaling Technology), anti-SOX2 (Abcam, Cambridge, MA), anti-OCT4 (Abcam), anti-Nanog (Abcam), anti-GAPDH (Abcam), and anti-MYCN (Abcam).