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2 protocols using 2 bromo 2 methylpropionyl bromide

1

Synthesis of PEGylated Nanoparticles for Drug Delivery

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2-Bromo-2-methylpropionyl bromide, triethylamine (TEA), N,N′-dicyclohexylcarbodiimide (DCC), 4-dimethylaminopyridine (DMAP), and copper(I) chloride (CuCl) were purchased from Alfa Aesar (Heysham, UK). Polyethylene glycol monomethyl ether (mPEG, Mw = 2000 g/mol) was obtained from TCI (Tokyo, Japan). N,N,N′,N″,N″-Pentamethyldiethylenetriamine (PMDETA), 2-hydroxyethyl disulfide, o-nitrobenzyl alcohol and succinic anhydride were purchased from Sigma-Aldrich (St. Louis, MO, USA). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) was from MP Biomedicals (Santa Ana, CA, USA). Phosphate buffer saline (PBS), Dulbecco’s Modified Eagle Medium (DMEM), Roswell Park Memorial Institute (RPMI) 1640 Medium, trypsin–EDTA, and fetal bovine serum (FBS) were purchased from Invitrogen (Waltham, MA, USA). Doxorubicin hydrochloride (DOX·HCl) was purchased from Combi-Blocks (San Diego, CA, USA).
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2

Synthesis of Graphene Oxide-Carbon Nanotube Composites

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Graphene oxide (GO) was provided by Promico CO., Ltd (Korea). Carbon nanotubes (CNTs) were provided by LG Chem. (Korea). Sulfur, 2-(dimethylamino)ethyl methacrylate (DMAEMA), iodoethane, hydroquinone, copper(i) bromide, N,N,N′,N′′,N′′-pentamethyldiethylenetriamine, poly(acrylic acid) (Mv = 450 000), poly(vinyl alcohol) (Mw = 9000–10 000, 80% hydrolyzed) were purchased from Aldrich and used as received. 2,2′-Azobis(isobutyronitrile) (AIBN) was purchased from Aldrich and recrystallized in ethanol before use. Carbon black, Super P® conductive, and 2-bromo-2-methylpropionyl bromide were purchased from Alfa Aesar and used as received. All the other reagents and solvents were obtained from reliable commercial sources and used as received.
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