A clinical strain ATCC 33277 of P. gingivalis (State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, China) was cultured on blood agar with hemin/menadione (Sigma-Aldrich Co., St. Louis, MO, USA) under anaerobic conditions. At the age of 7 weeks, the mice in P and V groups were orally inoculated with 100 μl phosphate buffered saline (PBS) containing 2% carboxymethylcellulose and 109 colony-forming units of live bacteria, 3 times at 2-day intervals within 5 days.10 (link),11 (link) The mice in N group received 100 μl PBS with 2% carboxymethylcellulose.10 (link),11 (link)
Hemin menadione
Manufactured by Merck Group
Sourced in United States, United Kingdom
Hemin/menadione is a laboratory product produced by Merck Group. It is a combination of the compounds hemin and menadione. Hemin is a ferric iron-containing porphyrin compound, and menadione is a synthetic vitamin K3 compound. The core function of this product is to serve as a laboratory reagent, but no further details on its intended use can be provided in an unbiased and factual manner.
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Lab products found in correlation
2 protocols using hemin menadione
1
Oral Inoculation of P. gingivalis in Mice
2
Oral Infection Model with Porphyromonas gingivalis
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All mice were randomly divided to infection and shame-infection group. Porphyromonas gingivalis (P. gingivalis) W50 was acquired from the State Key Laboratory of Oral Diseases, Sichuan University and cultured anaerobically in blood-agar (Oxoid, Oxoid Ltd., Hampshire, England) with hemin/menadione (Sigma-Aldrich).
At 6 weeks, the mice in the infection groups were inoculated with P. gingivalis as the following methods [17 (link)]: 109 colony-forming units of P. gingivalis were diffused in 100 μL phosphate buffered saline (PBS) with 2% carboxymethylcellulose and then orally inoculated three times every second day. The mice in shame-infection groups were infected with the equal volume of PBS (100 μL). All mice were killed at 18 week.
At 6 weeks, the mice in the infection groups were inoculated with P. gingivalis as the following methods [17 (link)]: 109 colony-forming units of P. gingivalis were diffused in 100 μL phosphate buffered saline (PBS) with 2% carboxymethylcellulose and then orally inoculated three times every second day. The mice in shame-infection groups were infected with the equal volume of PBS (100 μL). All mice were killed at 18 week.
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