Psma hbed cc

⁶⁸Gallium-HBED-CC was prepared using a GMP-grade ⁶⁸Ge-⁶⁸Ga-generator (GalliaPharm) in combination with an automated system (Modular-Lab Easy), cassettes and buffers as instructed by Eckert & Ziegler Eurotope (Berlin, Germany). 30μg PSMA-HBED-CC (ABX, Radeberg, Germany) was used per preparaton. Labelling quality control was performed by both instant thin layer chromatography and high performance liquid chromatography (Thermo Scientific Dionex UltiMate 3000), in combination with gamma detection. Images were acquired from skull vertex to the thighs using a TruePoint Biograph mCT40 scanner (Siemens, Erlangen, Germany), approximately 60 min after intravenous injection of 2 MBq/kg ⁶⁸Ga-HBED-CC-Glu-NH-CO-NH-Lys(Ahx) or 2 MBq/kg FDG in case of FDG-PET/CT imaging. A low dose CT scan was performed using Care Dose 4D and Care kV, reference parameters: 40 mAs, 120 kV. Subsequently, PET was acquired according to the European Association of Nuclear Medicine (EANM) recommendations with the following parameters: PET with time-of-flight and point spread function (TrueX) reconstruction, 4 iterations, 21 subsets, with a filter of 7.5 mm full width at half maximum [36 (link)].

A standard ⁶⁸Ge/⁶⁸Ga generator (Eckert and Ziegler) was used for elution of ⁶⁸Ga prior to labelling with PSMA-HBED-CC (ABX GmbH, Radeberg, Germany)^{22 (link),23 (link),34 (link)}. After injection of 129.0 ± 26.2MBq of ⁶⁸Ga-PSMA-HBED-CC, a low dose CT for attenuation correction (120 kVp, 30 mAs) and anatomical mapping was acquired within 89.0 ± 42.5 min immediately before the PET scan, using a Gemini TF 16 Astonish PET-CT scanner (Philips medical systems)^{35 (link)}. All 130 patients underwent a non-contrast-enhanced prostate multiparametric MRI at our institution at 3T (Magentom Skyra, Siemens Healthcare, Erlangen, Germany). Standard prostate MRI acquisition protocol included high resolution T2 weighted high resolution turbo spin echo sequences (T2 HR TSE, 25 slices, thickness 3 mm, gap 3 mm, TR 4040 ms, TE 116 ms, resolution: 0.47 × 0.47 mm), including DWI sequences (DWI, 25 slices, thickness 3 mm, gap 3 mm, TR 4800 ms, TE 58 ms, resolution 1.4 × 1.4 mm and b-factors 0/160/1200 s/mm²) with generation of an ADC map from b 0, b 160 and b 1200 images of the DWI. Native T1 high resolution isotropic volume sequences (T1 TSC, 49 slices thickness 5 mm, gap 6 mm, TE 11 ms, TR 800 ms, resolution 0.63 × 0.63 mm). No endorectal coils were used throughout all exams.

Elution of ⁶⁸Ga was performed using a standard ⁶⁸Ge/⁶⁸Ga generator (Eckert and Ziegler) [22 (link)]. Next, PSMA-HBED-CC (ABX GmbH, Radeberg, Germany) was labeled with ⁶⁸Ga [23 (link), 24 (link)].