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Primescript 2 first strand complementary dna synthesis kit

Manufactured by Takara Bio
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The PrimeScript II first-strand complementary DNA synthesis kit is a laboratory tool for generating complementary DNA (cDNA) from RNA samples. The kit provides the necessary reagents and enzymes to perform the reverse transcription process, which converts RNA into single-stranded cDNA molecules that can be used in various downstream applications.

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Lab products found in correlation

Sybr premix ex taq, by Takara Bio (1 mentions) Lightcycler 2.0 system, by Roche (1 mentions) Rnaprep pure cell bacteria kit, by Tiangen Biotech (1 mentions) Dnase 1, by Takara Bio (1 mentions) Trisure, by Meridian Bioscience (1 mentions)

Spelling variants of this product

PrimeScript kit (143 citations) PrimeScript II (29 citations) PrimeScript™ Synthesis kit (8 citations) PrimeScript II first-strand synthesis kit (2 citations) PrimeScript First Strand Synthesis Kit (2 citations) PrimeScript II First‐strand Complementary DNA (cDNA) Synthesis Kit (2 citations)

2 protocols using primescript 2 first strand complementary dna synthesis kit

1

Bacterial RNA Isolation and qPCR Analysis

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Cells were harvested in the exponential phase when the optical density at 600 nm reached 0.6–0.7 in sodium lactate broth medium. Total RNA was isolated by using an RNAprep pure Cell/Bacteria kit (TIANGEN, Beijing, China). Complementary DNA was obtained by using a PrimeScript II first-strand complementary DNA synthesis kit (TaKaRa), and qPCR was conducted with SYBR Premix Ex Taq (TaKaRa) on a LightCycler 2.0 system (Roche, Basel, Switzerland). The fold changes were determined by the 2−ΔΔCt method normalized to the 16S rRNA gene.
Liu L., Guan N., Zhu G., Li J., Shin H.D., Du G, & Chen J. (2016). Pathway engineering of Propionibacterium jensenii for improved production of propionic acid. Scientific Reports, 6, 19963.
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2

RNA Extraction and cDNA Synthesis

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Total RNA was isolated from Epstein-Barr virus-transformed lymphoblastoid cell lines using TRIsure (Bioline, London, UK) and treated with DNase I (Takara Bio, Shiga, Japan). Complementary DNA synthesis was obtained with the PrimeScript II first-strand complementary DNA Synthesis kit (Takara Bio) using oligo dT primers.
Uehara D.T., Hayashi S., Okamoto N., Mizuno S., Chinen Y., Kosaki R., Kosho T., Kurosawa K., Matsumoto H., Mitsubuchi H., Numabe H., Saitoh S., Makita Y., Hata A., Imoto I, & Inazawa J. (2016). SNP array screening of cryptic genomic imbalances in 450 Japanese subjects with intellectual disability and multiple congenital anomalies previously negative for large rearrangements. Journal of human genetics, 61(4).
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