Jcm 5700 scanning electron microscope

The hDPSCs were expanded further, and cells from 4 to 5th passages were seeded on one side of the prepared enamel discs after bleaching applications. The enamel discs were carefully placed inside appropriate wells of a 96 well plate containing 100 µL of prepared conditioned media. The cells were allowed to grow and reach approximately 80% confluence. Further, the cells were fixed using 2.5% glutaraldehyde. All samples were imaged using a JCM-5700 Scanning Electron Microscope (JEOL USA, Peabody, MA, USA).

Dried bacterial cultures were processed by suspending them in 3% glutaraldehyde of 0.1 M phosphate buffer for 1 h, pH 7.2. After washing three times, post-fixing was conducted in 1% osmium tetroxide (in H₂O) for 1 h. Afterward, the cells were dehydrated in 30%, 50%, 75%, 90%, and 2 × 100% for 5 min each. A 0.2-micron polycarbonate filter was used for purifying the dried bacteria, sucked by a vacuum pump in 100% ethanol [50 (link)]. Dried bacterial cultures were coated with metal stubs of the thin layer of heavy metal to increase the secondary electron signal (usually gold or gold palladium). The dried bacterial cultures were imaged in a JCM-5700 Scanning Electron Microscope (JEOL USA, Peabody, MA, USA), equipped with a mobile biological containment (Dycor Technologies Ltd., Edmonton, AB, Canada). Images of the dried bacterial cultures were taken after they had been processed under high vacuum at 6 kV, with an 8 mm working distance and a 30 μm objective lens aperture. A secondary electron detector was the tool used to collect the images, with which the acquisition time per image was 160 s, and each image was 2560 × 1920 pixels. Images of the ionic-liquid-stained samples were obtained using the above-noted settings, except that the acceleration voltage was adjusted to 4 kV. SEM images were recorded at magnifications ranging from 3000× to 20,000×.

All samples were imaged in a JCM-5700 Scanning Electron Microscope (JEOL USA, Peabody, MA, USA) contained inside a mobile biological containment enclosure (Dycor Technologies Ltd, Edmonton, AB, Canada)8 (link). Gold coated specimens were imaged under high vacuum at 6 kV, with an 8 mm working distance and a 30 μm objective lens aperture. Images were collected using the secondary electron detector, the acquisition time per image was 160 sec and each image was 2560 × 1920 pixels. Images of ionic liquid stained samples were obtained using the above noted settings with the exception that the acceleration voltage was adjusted to 4 kV. SEM images were recorded at magnifications ranging from 3,000 x to 20,000x.