Immunohistochemical (IHC) analysis was performed using antibodies against Ki-67, VEGF, CD31 (Cell Signaling Technology, USA) respectively. For immunofluorescence (IF) staining, sections were incubated with anti-CD56, anti-IFNγ, anti-TNF-α antibodies (Abcam, Cambridge, UK). Next, slides were mounted with Vectashield mounting media containing DAPI and were analyzed under fluorescence microscope.
Anti cd56
Manufactured by Abcam
Sourced in United States
Anti-CD56 is a laboratory antibody that binds to the CD56 protein, which is present on the surface of natural killer cells and some types of neurons. This antibody is commonly used in research applications to identify and study these cell types.
Automatically generated - may contain errors
Lab products found in correlation
Mantra system, by PerkinElmer (2 mentions)
Inform image analysis software, by PerkinElmer (2 mentions)
Pano 7 plex ihc kit, by Panovue (2 mentions)
Anti tnf α antibody, by Abcam (1 mentions)
Vascular endothelial growth factor (vegf), by Cell Signaling Technology (1 mentions)
Anti ifn γ, by Abcam (1 mentions)
Anti pd 1, by Abcam (1 mentions)
Dm2500, by Zeiss (1 mentions)
Dako antibody diluent, by Agilent Technologies (1 mentions)
Dako envision plus system, by Agilent Technologies (1 mentions)
Anti cd45, by Abcam (1 mentions)
Rhs 1, by Milestone (1 mentions)
Anti cd8, by Cell Signaling Technology (1 mentions)
Anti panck, by Abcam (1 mentions)
Ab108995, by Abcam (1 mentions)
Ab92511, by Abcam (1 mentions)
Ab177156, by Abcam (1 mentions)
5 protocols using anti cd56
1
Immunohistochemical and Immunofluorescence Staining
2
Tissue Microarray Analysis of HCC
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tissue microarrays containing 33 HCC tissue cores (2 mm in diameter, Supplementary Table S1 ) were ordered from the Shanghai Outdo biotech company (HLiv-HCC180Sur-05). For immunofluorescence double staining, sections were first deparaffinized and then incubated with retrieval solution (ZSGB-Bio, Beijing, China) for 15 min in a microwave oven. After treatment with 10% goat serum at 37 °C for half an hour, the tissues were incubated with anti-CD56 (Abcam, Cambridge, MA, USA) and anti-PD-1(Abcam) antibodies overnight. The sections were then incubated with FITC or PE-labeled secondary antibodies (ZSJQ-Bio). 4',6-diamidino-2-phenylindole was used for counterstaining. The staining was detected on a Zeiss DM2500 microscope with × 20 objectives.
3
Immunohistochemical Profiling of Tumor Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Paraffin-embedded blocks were sectioned (5 µm thickness) and transferred to silanized glass slides. The sections were then deparaffinised in xylene and rehydrated in a graded series of alcohol. Antigen retrieval was fulfilled by heating the sample in 0.01 M citrate buffer (pH 6.0) using a microwave vacuum histoprocessor (RHS-1; Milestone, Bergamo, Italy) at a final temperature of 121°C for 15 min. To block endogenous peroxidase activity, sections were blocked with 3% hydrogen peroxide in methanol for 10 min. Slides were incubated with mouse anti-CEACAM-1/CD66a (R&D Systems), anti-EpCAM (clone: MOC-31, Abcam), anti-CD45 (Abcam), and anti-CD56 (Abcam) antibodies, which were diluted to a ratio of 1:50 in Dako antibody diluent (Dako, Carpinteria, CA, USA) with background-reducing components at room temperature for 30 min. After washing, the Dako EnVision Plus system (Dako) was used at room temperature for 5 min. The immunoreaction was performed with diaminobenzidine for 5 min, followed by hematoxylin counterstaining. Immunofluorescence staining with confocal microscopy was performed as previously described with anti-CEACAM1 and anti-EpCAM antibodies.29 (link)
4
Multiplex Immunofluorescence Histology
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Antibodies such as anti-CD8 (CST70306, Cell Signaling Technology, United States), anti-CD56 (CST3576), anti-panCK (CST4545), anti-S100 (ab52642, Abcam, UK), anti-HLA-DR (ab92511), and anti-CD68 (BX50031, Biolynx, China) were used in mIF using the PANO 7-plex IHC kit (Panovue, Beijing, China), according to the manufacturer’s instructions. The Mantra System (PerkinElmer, Waltham, MA, United States) was used to scan the stained slides and reconstruct the images of the sections. inForm image analysis software (PerkinElmer, Waltham, MA, United States) was used to quantify the cells in the images.
5
Multiplexed Immunohistochemistry for Immune Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Thymidylate synthase (TS, TYMS) and ERO1A were stained using ab108995 and ab177156 antibodies (Abcam, UK), respectively, based on a two-step IHC protocol [15] . Positive TS expression was defined as the presence of nuclear and/or cytoplasm staining of tumor cells irrespective of their proportion or intensity. Positive ERO1A expression was determined according to IHC score [15] .
Multiplexed IHC (mIHC) and multispectral imaging mIHC and multispectral imaging were applied to detect immune cell subsets. Following the manufacturer's instructions, multiplex immunofluorescence staining was conducted based on PANO 7-plex IHC kit (Panovue, Beijing, China), with varied antibodies including anti-panCK (CST4545, Cell Signaling Technology, USA), anti-CD56 (CST3576), anti-CD8 (CST70306), anti-HLA-DR (ab92511, Abcam, UK), anti-S100 (ab52642) and anti-CD68 (BX50031, Biolynx, China). The invasive margin and tumor parenchyma were distinguished by S100 staining [16] .
The stained slides were scanned and a single stack image were built based on the Mantra System (PerkinElmer, Waltham, Massachusetts, US). After removing autofluorescence, images of sections were reconstructed according to a spectral library for multispectral unmixing and cells were quantified by the inForm image analysis software (Perki-nElmer, Waltham, Massachusetts, US).
Multiplexed IHC (mIHC) and multispectral imaging mIHC and multispectral imaging were applied to detect immune cell subsets. Following the manufacturer's instructions, multiplex immunofluorescence staining was conducted based on PANO 7-plex IHC kit (Panovue, Beijing, China), with varied antibodies including anti-panCK (CST4545, Cell Signaling Technology, USA), anti-CD56 (CST3576), anti-CD8 (CST70306), anti-HLA-DR (ab92511, Abcam, UK), anti-S100 (ab52642) and anti-CD68 (BX50031, Biolynx, China). The invasive margin and tumor parenchyma were distinguished by S100 staining [16] .
The stained slides were scanned and a single stack image were built based on the Mantra System (PerkinElmer, Waltham, Massachusetts, US). After removing autofluorescence, images of sections were reconstructed according to a spectral library for multispectral unmixing and cells were quantified by the inForm image analysis software (Perki-nElmer, Waltham, Massachusetts, US).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!