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Formic acid of hplc grade

Manufactured by Thermo Fisher Scientific
Sourced in China, United States

Formic acid of HPLC grade is a high-purity chemical reagent used in high-performance liquid chromatography (HPLC) applications. It is a colorless, volatile liquid with a characteristic pungent odor. Formic acid is a common mobile phase additive in HPLC, used to improve the separation and resolution of analytes.

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2 protocols using formic acid of hplc grade

1

Quantitative Analysis of Vancomycin

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Vancomycin hydrochloride was purchased from Dr. Ehrenstorfer GmbH (Augsburg,Germany). Nicotinamide adenine dinucleotide phosphate (NADPH) and phosphate buffer saline (PBS) were purchased from Shanghai Yisheng Biotechnology Co., Ltd (Shanghai, China) and Sangon Biotech Co., Ltd (Shanghai, China), respectively. Analytical grade magnesium chloride (MgCl2) was obtained from Yongda Chemical Reagent Co., Ltd (Tianjin, China). Acetonitrile and formic acid of HPLC grade were purchased from Thermo Fisher Scientific Co., Ltd (Shanghai, China) and DikmaPure Technologies Company (Beijing, China), respectively. HPLC grade methanol was purchased from Sunrise Chem, INC (New York, USA). Pure water was prepared by the Milli-Q system made in Millipore Corporation (Billerica, USA).
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2

Phytochemical Characterization of GLJ

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GLJ (batch No.: 20170726) was generously provided by Shanxi Guangyuyuan Traditional Chinese Medicine Co., Ltd. (Jinzhong, China). Standard references included kaempferol (≥98%, batch No.: MUST-17032911), psoralen (≥98%, batch No.: MUST-16092810), baohuoside Ⅰ (≥98%, batch No.: MUST-19042203), isobavachalcone (≥98%, batch No.: MUST-16102615), neobavaisoflavone (≥98%, batch No.: MUST-16092810), bavachinin A (≥98%, batch No.: MUST-18101705), oleanolic acid (≥98%, batch No.: MUST-17042706), ginsenoside Re (≥98%, batch No.: 180829), and ginsenoside Rg1 (≥98%, batch No.: 180917), which were obtained from Chengdu Must Biotechnology Co., Ltd. (Chengdu, China) and Shanghai Ronghe Pharmaceutical Technology Development Co., Ltd. (Shanghai, China). Methanol, petroleum ether, ethyl acetate, and n-butanol of analytical grade were purchased from Tianjin Four Fine Chemicals Co., Ltd. (Tianjin, China). Ultrapure water was prepared with a Milli-Q water purification system (Millipore, Bedford, MA, USA). Methanol of LC-MS grade and formic acid of HPLC grade were obtained from Thermo Fisher Scientific Inc. (Thermo Fisher Scientific, Waltham, MA, USA). Deuterated chloroform (CDCl3, 99.8% D) containing tetramethylsilane (TMS, 0.03%, m/V) and methanol-d4 (99.8% D) were obtained from J&K Scientific Ltd. (Beijing, China).
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