Cell morphology was investigated either under the light microscope (Leica MC170 HD) or a fluorescence microscope (Nikon). The viability of cells was analyzed with the LIVE/DEAD BacLight fluorescence stain from Life Technologies (Thermo Fisher Scientific). For all measurements, live cells were mounted onto 1% agarose pads supplemented with TB medium prior to microscopy analysis. Phase-contrast and fluorescence images were captured using a Ti eclipse inverted research microscope (Nikon) with a ×100/1.45 numerical aperture (NA) objective (Nikon). Image processing and cell length measurement was conducted by Fiji ImageJ 1.8.084 (link).
Live dead baclight fluorescence stain
Manufactured by Thermo Fisher Scientific
The LIVE/DEAD BacLight fluorescence stain is a two-color nucleic acid stain used to distinguish live and dead bacterial cells. It consists of a mixture of SYTO 9 green-fluorescent nucleic acid stain and the red-fluorescent nucleic acid stain, propidium iodide.
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Lab products found in correlation
2 protocols using live dead baclight fluorescence stain
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Cell Morphology and Viability Microscopy
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Cell Morphology and Viability Microscopy
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Cell morphology was investigated either under the light microscope (Leica MC170 HD) or a fluorescence microscope (Nikon). Viability of cells was analyzed with the LIVE/DEAD BacLight fluorescence stain from Life Technologies (Thermo Fisher Scientific). For all measurements, live cells were mounted onto 1% agarose pads supplemented with TB medium prior to microscopy analysis.
Phase-contrast and fluorescence images were captured using a Ti eclipse inverted research microscope (Nikon) with a 100x/1.45 numerical aperture (NA) objective (Nikon). Image processing and cell length measurement was conducted by Fiji ImageJ 1.8.0 (75) .
Phase-contrast and fluorescence images were captured using a Ti eclipse inverted research microscope (Nikon) with a 100x/1.45 numerical aperture (NA) objective (Nikon). Image processing and cell length measurement was conducted by Fiji ImageJ 1.8.0 (75) .
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