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Alexa 488 goat anti rabbit alexa 568 goat anti mouse

Manufactured by Thermo Fisher Scientific
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The Alexa Fluor 488 goat anti-rabbit and Alexa Fluor 568 goat anti-mouse are fluorescently labeled secondary antibodies used for detection in immunoassays and other fluorescence-based applications. The Alexa Fluor dyes provide bright, photostable labeling with minimal non-specific background. These reagents can be used for various types of fluorescence detection techniques.

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Lab products found in correlation

H 1000, by Vector Laboratories (2 mentions) Anti fibronectin and β catenin antibody, by Santa Cruz Biotechnology (2 mentions) Rabbit anti collagen antibody, by Santa Cruz Biotechnology (2 mentions) Rabbit anti ubc9 antibody, by Thermo Fisher Scientific (2 mentions) Vectashield mounting medium for fluorescence h 1000, by Vector Laboratories (1 mentions) Rabbit anti caveolin 1 antibody, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

IgG Alexa Fluor 488 or 568-conjugated goat anti-mouse or anti-rabbit antibodies Alexa 568 goat anti-rabbit Goat anti-mouse Alexa 568 Goat anti-rabbit Alexa 488 Goat anti-mouse Alexa 488 Anti-rabbit ALEXA 568 Alexa-568 anti-mouse 568 goat anti rabbit Alexa-488 anti-goat Alexa anti-rabbit 488

5 protocols using alexa 488 goat anti rabbit alexa 568 goat anti mouse

1

Immunofluorescence Analysis of Extracellular Matrix

Cited 1 time
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HCC1937, HCC1937 BRCA1, and CAL51 cells were cultured alone or transfected with pCDNA3, BRCA1a, BRCA1a Mut#1, for 24 hours in six-well plates onto glass cover slips overnight. The cells were washed and fixed in icy methanol for 5 minutes, and blocked using 10% BSA for 60 minutes, followed by primary polyclonal Rabbit anti-collagen antibody 1:250, primary polyclonal anti-fibronectin and β-catenin antibody (Santa Cruz), 1:500 diluted in 1.5% BSA made in PBS at 25°C for 1 hour and Alexa 488 goat anti-Rabbit/Alexa 568 goat anti-mouse (Molecular Probes) diluted in 1.5% BSA/PBS for 50 minutes and stained (Hoechst 33258, Pentahydrate, Life technologies). The cover slips were mounted with Vectashield mounting medium for fluorescence (H-1000 from Vector). The stained cells were examined by LSM 700 Confocal Microscope, equipped with 63X oil Ph immersion objectives. Composite filter cubes were used for the 488–405 as described previously [25 (link)].
Xu J., Olusola G., Footman A., Hansen N., Cheriyan A.M., Koganti K., Reddy V., Yezdani S., Eddy V., De’smond H., Bakinde N., Okoli J., Oprea G., Gundry K., Reddy E.S, & Rao V.N. (2019). A Provocative Molecular Link between Mammographic Density and BRCA1-loss associated TNBC. International journal of human genetics and genetic disorders, 1(1), 1-8.
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2

Immunofluorescence analysis of caveolin-1 and SIRT1

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MCF10A, HCC1937, HCC1937 BRCA1 and CAL51 cells were cultured alone or transfected with pCDNA3, BRCA1a, BRCA1a Mut#1, BRCA1a Mut#4, BRCA1a Mut#9 or EGFP-Antisense Ubc9 for 24 hours in six-well plates onto glass coverslips overnight. The cells were washed and fixed in icy methanol for 5 minute, and blocked using 10% BSA for 60 min, followed by primary polyclonal Rabbit anti-caveolin-1 antibody 1:150, primary polyclonal anti-SIRT1 antibody (Santa Cruz),l:150 diluted in 1.5% BSA made in PBS at 25°C for 1hr and Alexa 488 goat anti-Rabbit/Alexa 568 goat anti-mouse (Molecular Probes) diluted in 1.5% BSA/PBS for 50 min and stained (Hoechst 33258, Pentahydrate, Life technologies). The cover slips were mounted with Vectashield mounting medium for fluorescence (H-1000 from Vector). The stained cells were examined by LSM 700 Confocal Microscope, equipped with 63× oil Ph immersion objectives. Composite filter cubes were used for the 488–405 as described previously [27 (link)].
Xu J., Shumate C., Qin Y., Reddy V., Burnam Y., Lopez V., Okoli J., P Reddy E.S, & Rao V.N. (2017). A novel Ubc9 -dependent pathway regulates SIRT1- ER-α Axis and BRCA1-associated TNBC lung metastasis. Integrative molecular medicine, 4(4), 10.15761/IMM.1000298.
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3

Immunofluorescence Analysis of Ubc9 and BRCA1

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MCF10A, HCC1937 and UWB1.289 and UWB1.289 BRCA1 cells were cultured in six-well plates onto glass coverslips overnight. The cells were washed and fixed in icy methanol for 5 minute, and blocked using 10% BSA for 60 min, followed by primary polyclonal Rabbit anti-Ubc9 antibody 1:150, Monoclonal Mouse anti-BRCA1 antibody 1:100 diluted in 1.5 % BSA made in PBS at 25°C 1hr and Alexa488 goat anti-Rabbit/Alexa568 goat anti-mouse (Molecular Probes) diluted in 1.5% BSA/PBS for 50 min and stained (Hoechst 33258, Pentahydrate, Life technologies). The cover slips were mounted with Vectashield mounting medium for fluorescence (H-1000 from Vector). The stained cells were examined by LSM 700 Confocal Microscope, equipped with 63× oil Ph immersion objectives. Composite filter cubes were used for the 488–405 as described previously [41 (link)].
Xu J., Footman A., Qin Y., Aysola K., Black S., Reddy V., Singh K., Grizzle W., You S., Moellering D., Reddy E., Fu Y, & Rao V. (2016). BRCA1 Mutation Leads to Deregulated Ubc9 Levels which Triggers Proliferation and Migration of Patient-Derived High Grade Serous Ovarian Cancer and Triple Negative Breast Cancer Cells. International journal of chronic diseases & therapy, 2(3), 31-38.
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4

Immunofluorescence Analysis of Extracellular Matrix

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
HCC1937, HCC1937 BRCA1, and CAL51 cells were cultured alone or transfected with pCDNA3, BRCA1a, BRCA1a Mut#1, for 24 hours in six-well plates onto glass cover slips overnight. The cells were washed and fixed in icy methanol for 5 minutes, and blocked using 10% BSA for 60 minutes, followed by primary polyclonal Rabbit anti-collagen antibody 1:250, primary polyclonal anti-fibronectin and β-catenin antibody (Santa Cruz), 1:500 diluted in 1.5% BSA made in PBS at 25°C for 1 hour and Alexa 488 goat anti-Rabbit/Alexa 568 goat anti-mouse (Molecular Probes) diluted in 1.5% BSA/PBS for 50 minutes and stained (Hoechst 33258, Pentahydrate, Life technologies). The cover slips were mounted with Vectashield mounting medium for fluorescence (H-1000 from Vector). The stained cells were examined by LSM 700 Confocal Microscope, equipped with 63X oil Ph immersion objectives. Composite filter cubes were used for the 488–405 as described previously [25 (link)].
Xu J., Olusola G., Footman A., Hansen N., Cheriyan A.M., Koganti K., Reddy V., Yezdani S., Eddy V., De’smond H., Bakinde N., Okoli J., Oprea G., Gundry K., Reddy E.S, & Rao V.N. (2019). A Provocative Molecular Link between Mammographic Density and BRCA1-loss associated TNBC. International journal of human genetics and genetic disorders, 1(1), 1-8.
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5

Immunofluorescence Analysis of Ubc9 and BRCA1

Check if the same lab product or an alternative is used in the 5 most similar protocols
MCF10A, HCC1937 and UWB1.289 and UWB1.289 BRCA1 cells were cultured in six-well plates onto glass coverslips overnight. The cells were washed and fixed in icy methanol for 5 minute, and blocked using 10% BSA for 60 min, followed by primary polyclonal Rabbit anti-Ubc9 antibody 1:150, Monoclonal Mouse anti-BRCA1 antibody 1:100 diluted in 1.5 % BSA made in PBS at 25°C 1hr and Alexa488 goat anti-Rabbit/Alexa568 goat anti-mouse (Molecular Probes) diluted in 1.5% BSA/PBS for 50 min and stained (Hoechst 33258, Pentahydrate, Life technologies). The cover slips were mounted with Vectashield mounting medium for fluorescence (H-1000 from Vector). The stained cells were examined by LSM 700 Confocal Microscope, equipped with 63× oil Ph immersion objectives. Composite filter cubes were used for the 488–405 as described previously [41 (link)].
Xu J., Footman A., Qin Y., Aysola K., Black S., Reddy V., Singh K., Grizzle W., You S., Moellering D., Reddy E., Fu Y, & Rao V. (2016). BRCA1 Mutation Leads to Deregulated Ubc9 Levels which Triggers Proliferation and Migration of Patient-Derived High Grade Serous Ovarian Cancer and Triple Negative Breast Cancer Cells. International journal of chronic diseases & therapy, 2(3), 31-38.
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