The drug substance sulconazole nitrate was obtained from the USP Reference Standard. Commercial samples of sulconazole nitrate were purchased from Sigma-Aldrich (St. Louis, MI, USA), Spectrum Chemical (New Brunswick, NJ, USA), Glentham Life Sciences (Corsham, UK), and Erregierre (Sovere, Italy). Hydrochloric acid (37%), glacial acetic acid (≥ 99.7%), sodium hydroxide solution (10 N, J.T.Baker), trifluoroacetic acid (TFA, 99.5%, Acros Organics), ammonium acetate (LC/MS), methanol (LC/MS), and acetonitrile (HPLC and LC/MS) were purchased from Fisher Scientific (Waltham, MA, USA). Hydrogen peroxide (~30%) was obtained from Sigma-Aldrich. Water was purified with a Milli-Q plus system from Millipore (Bedford, MA, USA).
Sulconazole nitrate
Manufactured by Merck Group
Sourced in United States
Sulconazole nitrate is a synthetic antifungal agent. It is a white to off-white crystalline powder used in laboratory settings for research and testing purposes.
Automatically generated - may contain errors
Lab products found in correlation
Lc ms methanol, by Thermo Fisher Scientific (1 mentions)
Hydrochloric acid (hcl), by Thermo Fisher Scientific (1 mentions)
Hydrogen peroxide, by Merck Group (1 mentions)
Trifluoroacetic acid, by Thermo Fisher Scientific (1 mentions)
Milli q plus system, by Merck Group (1 mentions)
Glacial acetic acid, by Thermo Fisher Scientific (1 mentions)
Freestyle 293 expression medium, by Thermo Fisher Scientific (1 mentions)
Freestyle max reagent, by Thermo Fisher Scientific (1 mentions)
293f cells, by Thermo Fisher Scientific (1 mentions)
Nano glo vivazine substrate, by Promega (1 mentions)
2 protocols using sulconazole nitrate
1
Sulconazole Nitrate Characterization Protocol
2
Sulconazole Nitrate Modulation of Luciferase
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Sulconazole nitrate was from Sigma-Aldrich (catalog number 1623681). It was dissolved in anhydrous DMSO, and 1 mM stock solutions were stored at − 20 °C in aliquots. It was diluted with anhydrous DMSO further when desired. 293F cells (Thermo Fisher R79007) were grown and maintained in FreeStyle 293 Expression Medium (Thermo Fisher 12–338-018) at 37 °C 5% CO2. Cells were transiently transfected with plasmids using FreeStyle Max reagent (Thermo Fisher 16,447,100). Transfected cells were then incubated in medium containing biliverdin and/or Sulconazole nitrate (0–10 µM, 1% v/v DMSO; biliverdin concentrations above 10 µM hampered cell viability) for 3–24 h. Expression of nanoluciferase was measured by adding Nano-Glo Vivazine substrate (Promega N2580).
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