Nanog antibody

Manufactured by Abcam

Sourced in China

The Nanog antibody is a laboratory reagent used in research applications. It is a protein that specifically binds to and detects the Nanog transcription factor, which is a key regulator of embryonic stem cell pluripotency and self-renewal.

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Lab products found in correlation

Triton x 100, by Merck Group (1 mentions) Ssea 4, by Merck Group (1 mentions) Paraformaldehyde (pfa), by Merck Group (1 mentions) Alkaline phosphatase detection kit, by Merck Group (1 mentions) Tra 1 60, by Merck Group (1 mentions) Tra 1 81, by Merck Group (1 mentions) Ssea 1, by Merck Group (1 mentions) Dapi mounting medium, by Vector Laboratories (1 mentions) Fitc or cy3 conjugated secondary antibodies, by Merck Group (1 mentions) Oct3 4 antibody, by Santa Cruz Biotechnology (1 mentions) Iplabs software, by BD (1 mentions) Gelatin coated chamber slides, by Thermo Fisher Scientific (1 mentions) Anti mouse igg alexa 488 conjugate antibody, by Thermo Fisher Scientific (1 mentions) Gapdh antibody, by Proteintech (1 mentions) E cadherin, by Proteintech (1 mentions) N cadherin, by Proteintech (1 mentions) Vimentin, by Proteintech (1 mentions) Lgk974, by Selleck Chemicals (1 mentions) β catenin, by Proteintech (1 mentions) Cyclin d1, by Proteintech (1 mentions)

Spelling variants of this product

Nanog (153 citations) Anti-Nanog antibody (9 citations) Anti-Nanog antibody (rabbit polyclonal, (2 citations) Rabbit anti-NANOG antibody (2 citations) Anti-NANOG mouse polyclonal antibody (2 citations) Rabbit anti-human Nanog antibody (2 citations)

3 protocols using nanog antibody

Immunophenotyping of Human iPSCs

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AP staining was performed using the Alkaline Phosphatase Detection Kit (Sigma) according to the manufacturer’s instructions. For immunofluorescence assay, human iPSCs were adhered on slide chambers (Nunc) containing MEFs, fixed with 4% paraformaldehyde (Sigma) for 30 minutes at RT and permeabilized for 10 minutes with 1% TritonX-100 (Sigma). Cells were blocked with 5% BSA for 30 minutes at RT and incubated for one hour at RT or o/n at 4°C with NANOG antibody (Abcam), SOX2 (R&D), SSEA-1 (Chemicon), SSEA-4 (Chemicon), TRA1-60 (Chemicon) and TRA1-81 (Chemicon) diluted in PBS/TBS with 1% BSA. FITC- or Cy3-conjugated secondary antibodies (Sigma) diluted in the same solution was incubated for 1–1.5 hours at RT. Nuclei were counterstained with 1:4 dilution of DAPI mounting medium (Vector Labs). Samples were visualized under an inverted fluorescence microscope.

Rodriguez-Madoz J.R., San Jose-Eneriz E., Rabal O., Zapata-Linares N., Miranda E., Rodriguez S., Porciuncula A., Vilas-Zornoza A., Garate L., Segura V., Guruceaga E., Agirre X., Oyarzabal J, & Prosper F. (2017). Reversible dual inhibitor against G9a and DNMT1 improves human iPSC derivation enhancing MET and facilitating transcription factor engagement to the genome. PLoS ONE, 12(12), e0190275.

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Immunofluorescent Analysis of Pluripotency Markers

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Cells and blastocysts were plated on 0.1% gelatin-coated chamber slides (Thermo Fisher Scientific), treated as indicated, and then fixed with 3.7% formaldehyde diluted in PBS for 30 minutes. The slides were permeabilized with 0.1% Triton X-100 diluted in PBS, blocked with 10% BSA diluted in PBS, and incubated with an Oct3/4 antibody (Santa Cruz Biotechnology) and a Nanog antibody (Abcam) diluted in 5% BSA in PBS at a 1:200 dilution, for 2 hours. The slides were washed with PBS, and then were incubated with an anti-mouse IgG-Alexa 488 conjugate antibody (Thermo Fisher Scientific) and an anti-rabbit IgG-Alexa 548 conjugate antibody (Thermo Fisher Scientific) diluted in 5% BSA in PBS at a 1:400 dilution, for 1 hour. The slides were again washed with PBS and visualized by brightfield and fluorescent microscopy. All images were captured using IPLABS software (BD Biosciences) and processed using ImageJ software.

Hur Y.H., Feng S., Wilson K.F., Cerione R.A, & Antonyak M.A. (2020). Embryonic Stem Cell-Derived Extracellular Vesicles Maintain ESC Stemness by Activating FAK. Developmental cell, 56(3), 277-291.e6.

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Stem Cell Differentiation Protocol

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Fentanyl citrate was obtained from Northeast Pharmaceutical Group (China). The sources of reagents were as follows: Sox2, Oct4, N-cadherin, Vimentin, E-cadherin, β-catenin, GSK-3β, CD44, Cyclin D1, and GAPDH antibody (Proteintech, China); Nanog antibody (Abcam, USA); FUT8, c-Jun and VEGF antibody (Santa Cruz, USA); Lens culinaris agglutinin (LCA) Lectin, binding of α1, 6-fucosylation epitope. (Vector Laboratories, USA); p-GSK-3β (Ser⁹) antibody (Elabscience, China); LGK-974 (inhibitor of Wnt ligands) (Selleck, USA).

Yang H.F., Yu M., Jin H.D., Yao J.Q., Lu Z.L., Yabasin I.B., Yan Q, & Wen Q.P. (2017). Fentanyl Promotes Breast Cancer Cell Stemness and Epithelial-Mesenchymal Transition by Upregulating α1, 6-Fucosylation via Wnt/β-Catenin Signaling Pathway. Frontiers in Physiology, 8, 510.

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